鲤鱼汤对阿霉素肾病大鼠CD2相关蛋白及肾病蛋白的影响

目的:观察阿霉素肾病大鼠肾小球足细胞CD2相关蛋白(CD2-associated protein,CD2AP)和肾病蛋白(nephrin)的表达及鲤鱼汤对其表达的影响,进而探讨鲤鱼汤治疗肾病综合征的作用机制。方法:70只Wistar大鼠随机分为5组:正常对照组(N组)、模型组(M组)、福辛普利组(F组)、鲤鱼汤低剂量组(YD组)和鲤鱼汤高剂量组(YG组)。M组、F组、YD组和YG组大鼠采用尾静脉注射阿霉素6.5mg/kg复制阿霉素肾病模型。第3周开始进行干预,YG组和YD组分别予以高、低剂量鲤鱼汤(2.25、1.13g/mL)灌胃7周;F组予以0.09mg/mL福辛普利灌胃;N组和M组予以等量自来水灌胃。采用免疫组化方法检测各组大鼠肾脏中CD2AP、nephrin的表达,并计算其阳性表达的平均光密度值(AOD)。结果:与模型组相比,鲤鱼汤高剂量和鲤鱼汤低剂量组大鼠血清总蛋白水平、血清白蛋白水平等均有一定升高,尤其鲤鱼汤高剂量组差异均有统计学意义(P0.05);各治疗组肾小球中CD2AP与nephrin的表达较N组均降低,但与M组比较,都有不同程度的增加(P0.05)。结论:鲤鱼汤可不同程度地增强阿霉素肾病大鼠肾小球足细胞CD2AP和nephrin的表达,改善阿霉素肾病大鼠肾小球的损伤,进而起到治疗作用。

Effects of Cyprinus Carpio Decoction on the expression of nephrin and CD2AP in adriamycin nephropathy rats

Objective: By observing the expression of CD2AP and Nephrin on adriamycin nephritic rat kidney,and the effects of Cyprinus carpio decoction on it,to explore the mechanism of cyprinus carpio decoction on nephritic syndrome.Methods: 70 rats were randomly divided into 5 groups: normal control group(N group),model group(M group),Fosinopil group(F group),low Cyprinus carpio decoction group(YD group) and high Cyprinus carpio decoction group(YG group).The nephropathy model was induced by injecting adriamycin 6.5g/kg from caudas vein.The levels of CD2AP and nephrin expression were detected by immunohistochemistry method,and the average optical density(AOD) was calculated.Results: Compared with N group,the expression of CD2AP and nephrin decreased in treatment groups(P<0.05),especially in the M group;compared with the M group,the expression of CD2AP and nephrin increased in F group,YD group and YG group(P<0.05).Conclusion: The results of this study show that cyprinus carpio decoction play a importment role by improving the expression of CD2AP and nephrin on adriamycin nephropathy rats,and alleviating injury of glomerular filtration membrane in adriamycin nephropathy rats.