Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains |
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| Authors: | Cerna Jorge F Nataro James P Estrada-Garcia Teresa |
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| Affiliation: | Department of Molecular Biomedicine, CINVESTAV-IPN, Zacatenco, Mexico DF 07360, Mexico. |
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| Abstract: | We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC. |
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