Expression of Collagen Types I,II, IX,and X in the Mineralizing Turkey Gastrocnemius Tendon |
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| Authors: | Reginald W. Taylor Georgia K. Mitchell Jon Lee Andrade Kathy K. Svoboda |
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| Affiliation: | 1. Texas A&M College of Dentistry, Orthodontics, Dallas, Texas;2. Roseman University of Health Sciences, College of Dental Medicine, Henderson, Nevada;3. Carnegie Mellon University Mellon College of Science, Pittsburgh, Pennsylvania;4. Texas A&M College of Dentistry, Biomedical Sciences, Dallas, Texas |
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| Abstract: | The turkey gastrocnemius tendon mineralizes by intramembranous ossification with a transient chondrogenic phase. The mineralizing zone has hypertrophic chondrocytes similar to endochondral bone formation. These similarities prompted the evaluation of this tendon for the presence of type X collagen in the mineralizing zone. Tendons were removed, radiographed, decalcified, and embedded for frozen sections. Seral sections were H&E stained and immunostained individually with antibodies specific collagens (types I, II, IX, and X). Type I collagen was distributed widely throughout the mineralized tendon extracellular matrix. Types II and IX collagen were at the mineralized/non-mineralized junction. Type X collagen was in the pericellular matrix of hypertrophic chondrocytes and in some calcified matrix. These data support the theory that the gastrocnemius tendon has fibrocartilage characteristics and that type X collagen has a role in the tissue's mineralization. Anat Rec, 2019. © 2019 Wiley Periodicals, Inc. |
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| Keywords: | extracellular matrix collagen mineralization |
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