| 山楂中抑制HMG-CoA还原酶的活性成分及其协同效应的研究 |
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| 引用本文： | 黄文文,叶小利,李学刚,赵忠启,蓝萍,王亮,柳明,高英,朱家颖,李萍,冯平.山楂中抑制HMG-CoA还原酶的活性成分及其协同效应的研究[J].中国中药杂志,2010,35(18):2428-2432. |
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| 作者姓名： | 黄文文 叶小利 李学刚 赵忠启 蓝萍 王亮 柳明 高英 朱家颖 李萍 冯平 |
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| 作者单位： | 西南大学 药学院 药用资源研究所,重庆400716;国家海洋局 第三海洋研究所, 福建 厦门 361005;西南大学 生命科学学院,重庆400715;西南大学 药学院 药用资源研究所,重庆400716;西南大学 药学院 药用资源研究所,重庆400716;西南大学 药学院 药用资源研究所,重庆400716;西南大学 药学院 药用资源研究所,重庆400716;西南大学 药学院 药用资源研究所,重庆400716;西南大学 药学院 药用资源研究所,重庆400716;西南大学 药学院 药用资源研究所,重庆400716;北碚中医院, 重庆 400700;北碚中医院, 重庆 400700 |
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| 基金项目： | 重庆市科委攻关项目 ;重庆市卫生局重点项目 |
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| 摘 要： | 目的:研究山楂降血脂活性成分及作用机制。方法:以HMG-CoA还原酶活性为靶标,采用大孔树脂和硅胶等进行分离纯化,筛选HMG-CoA还原酶抑制剂。结果:获得4个对HMG-CoA还原酶活性具有较强抑制作用的化合物,为槲皮素、金丝桃苷、芦丁和绿原酸,4个化合物抑制率之和为50.01%,复配后的抑制活性为79.48%。结论:槲皮素和金丝桃苷为山楂中抑制HMG-CoA还原酶活性的主要活性成分,且单体之间存在协同作用。
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| 关 键 词： | 山楂 3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMG-CoA还原酶) 协同作用 槲皮素 金丝桃苷 |
| 收稿时间： | 2009/10/9 0:00:00 |
The inhibition activity of chemical constituents in hawthorn fruit and their synergistic action to HMG-CoA reductase |
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| HUANG Wenwen,YE Xiaoli,LI Xuegang,ZHAO Zhongqi,LAN Ping,WANG Liang,LIU Ming,GAO Ying,ZHU Jiaying,LI Ping and FENG Ping.The inhibition activity of chemical constituents in hawthorn fruit and their synergistic action to HMG-CoA reductase[J].China Journal of Chinese Materia Medica,2010,35(18):2428-2432. |
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| Authors: | HUANG Wenwen YE Xiaoli LI Xuegang ZHAO Zhongqi LAN Ping WANG Liang LIU Ming GAO Ying ZHU Jiaying LI Ping and FENG Ping |
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| Institution: | Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, China;School of Life Science, Southwest University, Chongqing 400715, China;Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Institute of Pharmaceutical Resources, School of Pharmaceutical Science, Southwest University, Chongqing 400716, China;Beibei Traditional Chinese Medicine Hospital, Chongqing 400700, China;Beibei Traditional Chinese Medicine Hospital, Chongqing 400700, China |
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| Abstract: | Objective : To study the hypolipidemic active compounds from Crataegus pinnatifida and mechanism of action of those. Method : Guided by the inhibitory activity to HMG-CoA reductase, the active compounds were separated and purified with macroporous resin and silica gel. Result : Four active compounds were obtained, which were quercetin, hyperoside, rutin and chlorogenic acid, the sum of their inhibitory rate was 50.01%, and the total inhibitory rate of the mixture of four active compounds matched was 79.48%. Conclusion : Quercetin and hyperoside were the principle active components inhibiting HMG-CoA reductase in Hawthorn fruit, and there were synergistic action among them. |
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| Keywords: | hawthorn fruit 3-hydroxy-3-metheylglutaryl coenzyme A reductase (HMG-CoA reductase) synergistic action quercetin hyperoside |
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