幽门螺杆菌感染与胃粘膜上皮p53蛋白表达的关系

目的 研究幽门螺杆菌（HP）根除治疗对p53蛋白表达的影响，并探讨HP与胃癌的关系。方法 利用免疫组化染色对206例胃粘膜活检组织（其中包括正常胃粘膜、HP阴性胃癌前各阶段病变和HP阳性胃癌前各阶段病变HP根除治疗前后及胃癌）进行p53蛋白表达的分析。结果 在正常胃粘膜未见p53蛋白表达。HP阳性胃癌前阶段病变p53蛋白表达阳性率显著高于正常胃粘膜及HP阴性胃癌前阶段病变组，低于HP阳性异型增生组。HP阳性胃癌前阶段病变HP根除治疗后，其p53蛋白表达阳性率显著降低。结论 HP感染可促进突变型p53基因表达增强；HP根除治疗可使胃癌前阶段病变p53蛋白表达阳性率明显降低。

Relationship between Helicobacter Pylori Infection and p53 Protein Expression in Gastric Epithelium

Objective To investigate the effect of the eradication of Helicobacter pylori (HP) on p53 protein expression, and understand the relationship between HP and gastric carcinoma. Methods p53 protein immunohistochemical staining were used in the research. Total 206 gastric mucosal biopsy specimens, including normal gastric mucosa, HP-negative gastric precancerous lesions, HP-positive gastric precancerous lesions before and after eradication of HP and gastric carcinoma, were studied. Results p53 protein positive expression was not found in normal gastric mucosa. Positivity of p53 protein expression in HP-positive gastric precancerous lesions was significantly higher than in normal gastric mucosa and in HP-negative gastric precancerous lesions; Positivity of p53 expression in HP-positive metaplasia was significantly higher than in HP-negative metaplasia, and lower than in HP-positive dysplasia; After eradication of HP, positivity of p53 expression significantly decreased in HP-positive gastric precancerous lesions. Conclusion After eradication of HP positivity of p53 expression in gastric precancerous lesions significantly decreased. It was supposed that HP infection caused mutated p53 gene expression to increase.