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| 细胞培养法评价含硅羟基磷酸钙涂层镁合金的生物相容性 | |
| 引用本文： | 王佐周,潘锋,张二林.细胞培养法评价含硅羟基磷酸钙涂层镁合金的生物相容性[J].解剖科学进展,2011,17(1):40-43. |
| 作者姓名： | 王佐周 潘锋 张二林 |
| 作者单位： | 1. 中国医科大学病理学教研室,辽宁,沈阳,110001 2. 中国医科大学人体解剖学教研室,辽宁,沈阳,110001;中国医科大学组织工程研究所生物材料研究室,辽宁,沈阳,110001 3. 中国科学院沈阳金属研究所,辽宁,沈阳,110016 |
| 基金项目： | 国家自然科学基金资助项目(No.81000791); 辽宁省教育厅高校基金资助项目(No.L2010580) |
| 摘 要： | 目的 采用细胞培养法在体外评估镁合金表面不同硅含量的羟基磷酸钙涂层对成骨细胞的毒性反应.方法 采用经鉴定的SD大鼠成骨细胞接种于三种含有不同浓度硅含量的羟基磷酸钙涂层的镁合金及对照组裸镁合金,MTT法检测细胞活力并榆测成骨细胞的碱性磷酸酶表达;扫描电镜观察细胞生长情况.结果 细胞活力检测和碱性磷酸酶活性分析均表明,硅含... |
| 关 键 词： | 成骨细胞 细胞培养法 镁合金 含硅羟基磷酸钙涂层 SD大鼠 |
Compatibility evaluated by cell culture assay on Si-HA coating of magnesium alloy |
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| WANG Zuo-zhou,PAN Feng,ZHANG Er-lin.Compatibility evaluated by cell culture assay on Si-HA coating of magnesium alloy[J].Progress of Anatomical Sciences,2011,17(1):40-43. | |
| Authors: | WANG Zuo-zhou PAN Feng ZHANG Er-lin |
| Institution: | WANG Zuo-zhou1,PAN Feng2,3,ZHANG Er-lin4 (1. Department of Pathology,2.Department of Human Anatomy,3.Biomaterial Research Laboratory of Institute of Tissue Engineering,China Medical University,Liaoning Shenyang 110001 China,4. Shenyang Institute of Metal Research,Chinese Academy of Sciences,Liaoning Shenyang 110016 China) |
| Abstract: | Objective To evaluate the cytotoxicreaction of calcium hydroxyapatite coating with different silicon concentration of Mg-Mn-Zn-Ca. Methods The identified osteoblasts of SD rats were seeded on calcium hydroxyapatite coating with three kinds of different silicon concentration of Mg-Mn-Zn-Ca and naked ones. The cell vitality was detected with MTT assay, and alkaline phosphatase(ALP) vitality was measured by ALP detection kit. The growth of osteoblasts on different concentrations of coatings and naked magnesium... |
| Keywords: | osteoblast cell culture assay magnesium alloy Si-HA coating SD rat |
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