内毒素性急性肺损伤大鼠肺组织p38 mRNA及其蛋白质表达的变化

目的探讨内毒素性急性肺损伤 (ALI)大鼠肺组织p38mRNA及其磷酸化蛋白质表达的变化。方法静脉注射脂多糖 (LPS)复制大鼠ALI模型。分别采用原位分子杂交和蛋白质印迹方法检测肺组织 p38mRNA及其磷酸化蛋白质的表达 ,并观察肺组织病理学改变。结果正常大鼠肺组织有少量p38mRNA表达 ,可见于平滑肌细胞、内皮细胞、肺泡上皮细胞和肺泡巨噬细胞。静脉注射LPS 2小时能成功复制ALI模型 ,PaO2 显著下降 (p <0 .0 1) ,p38mRNA及其磷酸化蛋白质表达显著增加 ,分别为正常对照组的 2 .34和 2 .74倍 (p <0 .0 1)。通过直线相关分析发现 ,PaO2 与肺组织 p38mRNA和磷酸化p38MAPK表达之间分别呈显著负相关 (r=- 0 .6 5 2 ,- 0 .713,p均 <0 .0 1)。结论内毒素性肺损伤肺组织 p38mRNA及其磷酸化蛋白质的表达均上调 ,可能与ALI的病理生理过程有关。

Changes of p38 mRNA and p38 Protein in Rat Lung with Lipopolysaccharide(LPS)-induced Acute Lung Injury

Objective To approach the changes of p38 mRNA and p38 prote in in rat lung with LPS-induced acute lung injury (ALI). Mothods LPS was injected intravenously to prepare ALI model. By using in situ hybridiz ation staining and Western blot, p38 mRNA and phospho-p38 protein were measured respectively . Histopathologic changes in lung was observed.Results In control group rats, a little p38 mRNA was expressed in smooth muscle cells, endothelial cells, alveolar epithelial cells and alveolar macrophages. Tw o hours after LPS injected, PaO 2 was decreased (p<0.01) and ALI model was prep ared successfully. Both the expression of p38 mRNA, mainly in the infiltrated in flammatory cells, and phospho-p38 protein were increased significantly(p<0.01), There were good negative correlations between the PaO 2 and the expression of both p38 mRNA and phospho-p38 protein (r=-0.652,-0.713,p<0.01). Conclusion The expression of both p38 mRNA and phospho-p38 protein is up-regulated in rat lung with LPS-induced ALI. This may be related to t he pathophysiologic process of ALI.