近红外光谱—偏最小二乘方法同时测定复方磺胺甲唑片的两种有效成分

目的建立复方磺胺甲口恶唑片的两种有效成分磺胺甲口恶唑(SMZ)和甲氧苄啶(TMP)的快速同时测定方法。方法基于近红外漫反射光谱技术,利用偏最小二乘方法建立该复方中SMZ和TMP的定量分析多元校正模型。结果对于所建立的SMZ与TMP模型,相关系数分别为:100.00%与100.00%;校正集残差分别为:0.0163与0.008 36;预测均方差分别为:0.156与0.0815。结论本方法在样品不经任何预处理的情况下,实现了该制剂的两种有效成分SMZ和TMP的简单、快速、两组分同时准确测定。

Simultaneous analyses of the two effective components in compound sulfamethoxazole tablets by near-infrared spectroscopy and partial least square method

Objective Build a rapid and simultaneous analytical method for the two effective components,sulfamethoxazole(SMZ) and trimethoprim(TMP) contained in compound sulfamethoxazole tablets.Methods Based on near-infrared diffuse reflection spectroscopy,the multivariate calibration models for SMZ and TMP in the compound were built using partial least squares(PLS) method.Results For the models,the parameters were 100.00% and 100.00% of the correlation coefficients,0.0163 and 0.008 36 of the root mean square errors of cross-validation(RMSECV) for the calibration sample sets,and 0.156 and 0.0815 of RMSECV for prediction sample sets,respectively for SMZ and TMP.Conclusion Without any pre-processing for the measured drug samples,the method could carry out simple,rapid,simultaneous and accurate analyses for SMZ and TMP in the compound tablet.