| ConA刺激小鼠T淋巴细胞早期事件研究 |
| |
| 引用本文： | 冯元怡,程伯基,林克椿.ConA刺激小鼠T淋巴细胞早期事件研究[J].北京大学学报(医学版),1989(2). |
| |
| 作者姓名： | 冯元怡 程伯基 林克椿 |
| |
| 作者单位： | 北京医科大学生物物理学教研室
(冯元怡，程伯基)，北京医科大学生物物理学教研室(林克椿) |
| |
| 摘 要： | 本文研究了刀豆蛋白A(ConA)刺激小鼠脾脏T淋巴细胞的早期事件。表明ConA可引起T细胞胞质游离Ca~(2+)浓度的快速显著上升、膜电位的轻微超极化和膜流动性的增大。在膜电位和膜脂流动性的变化中Ca~(2+)均起一定作用,提示Ca~(2+)是ConA刺激T淋巴细胞活化的早期事件中的关键物质。
|
| 关 键 词： | 早期事件 胞质游离钙离子 跨膜电位 膜流动性 |
STUDY OF EARLY EVENTS IN MOUSE SPLEEN T LYMPHOCYTES STIMULATED BY CONCANAVALIN A |
| |
| Institution: | Department of Biophysics Feng Yuanyi,et al |
| |
| Abstract: | The early events occurring in mouse spleen T lymphocytes by stimulation of concanavalin A (Con A) have been investigated. It was observed that Con A evoked a rapid increase in cytoplasmic free Ca2+ level, and it was also shown that the increase in Ca2+]| induced by Con A stimulation was due to both increased Ca2+ transmembrane influx and the release of Ca2+ from intracellular calcium stores. Addition of Con A induced a slight hyperpolarization in T cell membrane potential, but A23187, a calciumionion ionophore, induced significant depolarization. Membrane fluidity of T lymphocytes increased after the cells were exposed to Con A, but no change in membrane fluidity was observed in Ca2+ free medium. These results imply that Ca2+ is one of the key factors in early events of T lymphocytes activation stimulated by Con A. |
| |
| Keywords: | Early event Cytoplasmic free Calcium ion Transmembrane potential Membrane fluidity |
| 本文献已被 CNKI 等数据库收录！ |
