B淋巴细胞刺激因子及其受体BAFF-R在系统性红斑狼疮患者外周血单个核细胞中的表达及意义

目的 探讨B淋巴细胞刺激因子(Blys)及其受体BAFF-R在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中的表达情况及临床意义.方法 应用流式细胞仪技术,检测90例SLE患者PB-MCs中Blys及其受体BAFF-R的表达,并与45例健康志愿者检测结果 进行比较.分析Blys及BAFF-R的表达与SLE病情活动、其他实验指标及自身抗体的关系.结果 ①SEE患者PBMCs中Blys及BAFF-R的表述百分率比健康对照组高(均P<0.001).其中,活动期和稳定期SLE患者Blys和BAFF-R的表达率均高于健康对照组(P<0.001或0.01);活动期和稳定期比较,Blys的表达较高(P<0.05),而BAFF-R的表达无统计学差异.②SLE患者PBMCs中Blys的表达水平与SLE疾病活动指数评分呈正相关(r=0.728,P<0.001),与免疫球蛋白IgG、IgM里正相关(r=0.691,P<0.001和r=0.453,P<0.01),与补体C3、C4呈负相关(r=-0.510,P<0.001和r=-0.312,P<0.05).③抗dsDNA抗体(+)组患者PBMCs中Blys的表达较 dsDNA抗体(-)组高(P<0.01).Clq抗体(+)组患者PBMCs中的表达也较C1q抗体(-)组高(P<0.01).结论 Blys及BAFF-R在SLE外周血单个核细胞中的表达率升高.Blys的表达在一定程度上反映病情的活动,并与自身抗体的产生相关.Blys及其特异性受体BAFF-R可能参与SLE的发病.

Expression and significance of B lymphocyte stimulator and BAFF-receptor in peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

Objective To investigate the expression and significance of B lymphocyte stimulator (Blys) and its receptor BAFF (BAFF-R) in peripheral blood mononuclear cells (PBMCs) from patients with systemic lupus erythematosus (SLE). Methods The expression of Blys and BAFF-R was measured by flow cytometry in 90 pa-tients with SLE,which was compared with that of 45 healthy controls. The relationships between the expression of Blys, BAFF-R and other laboratory parameters as well as disease activity were analyzed. Results The expression of Blys and BAFF-R in PBMCs from patients with SLE was significantly elevated compared to healthy controls (P <0.001), so did the active group (P < 0.001) and inactive group (P < 0.001 and P < 0.01). The expression of Blys in PBMCs from active SLE patients was higher than that of inactive patients (P <0.05). However,there was no statisti-cal difference of BAFF-R between the two groups. The expression of Blys in PBMCs was positively related to SLEDAI (r =0.728,P <0.001) ,IgG and IgM(r=0.691,P<0.001 and r =0.453,P<0.01) ,but negatively related to C3 and CA (r = -0.510, P < 0.001 and r = -0.312, P < 0.05). The expression of Blys in dsDNA positive group was higher than those of dsDNA negative group (P < 0.01). The expression of Blys and BAFF-R in Cl qAb positive group was higher than those of ClqAb negative group as well (P <0.01). Conclusion The expression of Blys and its receptor BAFF-R in PBMCs from SLE is elevated ,which may reflect the disease activity and is related to the pro-duction of autoantibody. They might be involved in the pathogenesis of SLE.