广金钱草多糖的提取工艺及其体外抗氧化活性研究

目的研究广金钱草多糖的水提工艺条件及其体外抗氧化活性。方法以提取温度(A)、料液比(B)、提取时间(C)为条件,建立三因素三水平的正交试验,研究广金钱草多糖的水提工艺;体外抗氧化活性的研究,是以邻二氮菲-Fe2+-H2O2体系研究多糖对羟自由基(-OH)的抑制作用,以邻苯三酚自氧化法研究多糖对超氧阴离子自由基(O-2)的抑制作用;多糖的含量测定方法采用苯酚-硫酸法。结果广金钱草多糖水提工艺的最佳条件为温度100℃,料液比为1∶20,提取时间3.5 h,提取3次。广金钱草多糖对-OH、O-2有明显的清除作用。结论从广金钱草中提取的多糖具有清除-OH、O-2的抗氧化作用。

Extraction technology and antioxidant activity in vitro of desmodium styracifolium polysaccharides

Objective To study the desmodium styracifolium polysaccharides water extraction process conditions and the antioxidant activity in vitro. Methods The extraction temperature （A）, ratio of material to liquid（ B ）, extraction time （C） as the condition to establish the three factors and three levels orthogonal test to study the water extracting technology and antioxidant activity in vitro of desmodium styracifolium polysaccharides. The phenanthroline-Fe^2＋ -H2O2 system was used to study the inhibitory action of polysaccharides to hydroxyl radical（-OH） ;the pyrogallol autoxidation method was used to study the inhibitory action of polysaccharides to superoxide anion（ O2^- ） ;the phenol-sulphuric acid method was used to detect the contents of polysaccharide. Results The optimum conditions of desmodium styracifolium polysaccharides water extraction as follow： 100 ~C of temperature, solid to liquid ratio 1 ： 20, the extraction time 3.5 h for 3 times. The desmodium styraeifolium polysaccharides had apparente scavenging effect on -OH and O2^- Conclusion Polysaccharides extracted from desmodium styracifolium has a effect of antioxidant on -OH and O2^-.