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宫内感染后低龄大鼠脑组织中胶质纤维酸性蛋白的表达变化
引用本文:袁天明,俞惠民,顾伟忠,汤宏峰,李建平.宫内感染后低龄大鼠脑组织中胶质纤维酸性蛋白的表达变化[J].中国病理生理杂志,2004,20(12):2284-2286.
作者姓名:袁天明  俞惠民  顾伟忠  汤宏峰  李建平
作者单位:1. 浙江大学医学院附属儿童医院新生儿科, 浙江 杭州 310003;
2. 浙江大学医学院附属儿童医院病理科, 浙江 杭州 310003;
3. 浙江大学医学院附属儿童医院检验科, 浙江 杭州 310003
基金项目:浙江省自然科学基金资助项目(No.302653),浙江省科技计划项目(No.2003C33050)
摘    要:目的:探讨胶质纤维酸性蛋白(GFAP)在宫内感染后低龄大鼠脑组织中的表达变化及其意义。方法: 对孕大鼠子宫内注入大肠杆菌建立宫内感染的大鼠模型,以子宫内注入生理盐水为对照组。两组分别于生后1、3、7、14及21 d取幼鼠脑组织,应用免疫组化方法检测脑组织中不同脑区GFAP的表达。结果: 生后1、3 d龄大鼠仅脑室旁白质区可见少许GFAP阳性细胞,两组细胞数无显著差异(P>0.05),其余脑区未见明显GFAP表达。感染组7日龄大鼠脑室旁白质和海马区GFAP阳性细胞数增多,与对照组比较差异显著(脑室旁白质区:9.73±3.55 vs 5.67±1.90,P<0.05;海马区:7.81±3.61 vs 2.16±1.11,P<0.05)。感染组14 d龄大鼠脑室旁白质、胼胝体及皮层区GFAP阳性细胞数增多,与对照组比较均有显著差异(脑室旁白质区:12.72±1.81 vs 9.00±0.93,P<0.01;胼胝体区:10.98±3.26 vs 4.44±1.15,P<0.01;皮层区:5.43±1.79 vs 2.71±0.67,P<0.01)。两组21 d龄大鼠各脑区GFAP阳性细胞数无显著差异(P>0.05)。结论: 宫内感染后低龄大鼠脑组织中GFAP表达增加。

关 键 词:宫内感染  神经胶质原纤维酸性蛋白质    大鼠  
文章编号:1000-4718(2004)12-2284-03
收稿时间:2003-05-27

Expression of glial fibrillary acidic protein in developing rat brain after intrauterine infection
YUAN Tian-ming,YU Hui-min,GU Wei-zhong,TANG Hong-feng,LI Jian-ping.Expression of glial fibrillary acidic protein in developing rat brain after intrauterine infection[J].Chinese Journal of Pathophysiology,2004,20(12):2284-2286.
Authors:YUAN Tian-ming  YU Hui-min  GU Wei-zhong  TANG Hong-feng  LI Jian-ping
Institution:1. Department of Neonatology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;
2. Department of Pathology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;
3. Laboratory, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
Abstract:AIM: To investigate the alteration of glial fibrillary acidic protein (GFAP) immunoreactivity in developing rat brain after intrauterine infection. METHODS: Escherichia coli (E.coli) was inoculated into uterine horn of pregnant rats when gestation was 15 days and the control group was inoculated with normal saline. Immunohistochemistry was used for evaluation of GFAP expression in pup brains at postnatal day 1 (P1), P3, P7, P14, P21. RESULTS: GFAP-immunopositive cells was scarce in the periventricular white matter at P1 and P3 in two groups (P>0.05), but not in other brain regions. The number of GFAP-immunopositive cells of the E.coli-treated pups was markedly increased in periventricular white matter and hippocampus at P7 compared with the control group (P<0.05). The E.coli-treated pups at P14 showed a marked increase in GFAP expression in periventricular white matter, corpus callosum and cortex (P<0.01). However, no significant different levels of GFAP expression in any brain regions were found at P21 between two groups (P>0.05). CONCLUSION: Intrauterine infection induces an increased expression of GFAP in the neonatal brain.
Keywords:Intrauterine infection  Glial fibrillary acidic protein  Brain  Rats
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