细胞周期调控因子cyclinD1、p27、p16在大肠癌中的表达

目的 通过分析大肠癌中cychn D1、p27和p16的表达情况,以及它们与临床病理特征的关系,探讨三者在大肠癌发生、发展中的作用及意义.方法 采用免疫组织化学一步法,分别检测cyclinD1、p27和p16在53例大肠癌组织(大肠癌组)和30例正常大肠黏膜(对照组)中的表达情况,并进行比较.结果 大肠癌组和对照组中cyclinD1阳性表达率分别为64.15%(34/53)、6.67%(2/30);p27阳性表达率分别为30.19%(16/53)、76.67%(23/30);p16阳性表达率分别为43.40%(23/53)、83.33%(25/30).两组比较差异均有统计学意义(P<0.01).中高分化大肠癌患者与低分化大肠癌患者比较,三项指标的阳性表达率差异均有统计学意义(P<0.05).大肠癌Dukes分期A+B期与C+D期cyclinD1、p27和p16阳性表达率比较差异均有统计学意义(P<0.05).结论 cyclinD1、p27和p16与大肠癌的发生及恶性程度有关,可作为评价大肠癌恶性程度的重要指标,并为大肠癌患者临床生物学行为和预后评价提供重要参考.

The protein expression of cyclinD1, p27 and p16 in the colorectal carcinoma

Objective To investigate the effect and significance of cyclinD1, p27 and p16 in the genesis and progress of the colorectal carcinoma by analyzing their expression and their relation with clinicopathological parameters. Methods Immunohistochemistry one-step method was used to examine the expression of cyclinD1, p27 and p 16 respectively in 53 cases of colorectal carcinoma tissues and 30 cases of normal colorectal tissues. SPSS13.0 statistic software was used to analyze the correlation of their abnormal expressions to colorectal carcinoma. Results The positive expression rate of cyclinD1 was 64.15% (34/53) in the colorectal carcinoma tissues and 6.67% (2/30) in the normal colorectal tissues (P < 0.01). The positive expression rate of p27 was 30.19% (16/53) in the colorectal carcinoma tissues and 76.67% (23/30) in the normal colorectal tissues (P < 0.01). The positive expression rate of p 16 was 43.40% (23/53) in the colorectal carcinoma tissues and 83.33%(25/30) in the normal colorectal tissues (P < 0.01). Compared moderately and well differentiated carcinoma with poor differentiated carcinoma, each of these three indicators had significant difference (P <0.05). Compared with A+B stage and C+D stage in Dukes stage of colorectal carcinoma, each of these three indicators had significant difference(P < 0.05). Conclusions The changes of cyclinD1, p27 and p16 are related to the occurrence and malignant degree of colorectal carcinoma which can provide an important reference to the clinical biology manifestation and prognosis of the patients suffering from colorectal carcinoma.