降糖宁胶囊中人参皂苷Rgl、Re的含量方法学考察 |
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引用本文: | 刘莉,齐洋.降糖宁胶囊中人参皂苷Rgl、Re的含量方法学考察[J].黑龙江医药,2014,0(2):304-306. |
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作者姓名: | 刘莉 齐洋 |
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作者单位: | 刘莉(哈尔滨圣泰生物制药有限公司);齐洋(哈尔滨圣泰生物制药有限公司); |
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摘 要: | 目的:建立HPLC法测定降糖宁胶囊中人参含量的方法。方法:Packed ODS-A C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05%磷酸溶液(100:400)为流动相;检测波长为203nm,流速为1ml/min。结果:人参皂苷Rg1、人参皂苷Re进样量均在0.4μg~1.6μg范围内进样量与峰面积呈良好线性关系(r=0.9996);方法的平均回收率:人参皂苷Rg1为98.68%,RSD为1.71%(n=9);人参皂苷Re为100.81%,2.71%(n=9)。结论:通过采用本方法对降糖宁胶囊人参中(Rg1、Re)的含量进行测定,方法准确可行,灵敏度高,能够有效控制产品质量。
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关 键 词: | 降糖宁 Rgl Re HPLC |
Methodological Study on Ginsenoside Re in Contents of Ginsenoside Rgl,Jiangtangning Capsules |
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Institution: | Liu Li, et al (Harbin Sanctity Pharmaceutical Co., Ltd.) |
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Abstract: | 0bjective:To establish the determination methods of ginsenoside Rg1, ginsenoside Re In Jiangtangning capsules by HPLC. Methods:A Packed ODS-A Cl8 column(4.6mm ×250mm,5μm), Acetonitrile-0.05%phosphoric acid solution (100:400) as the mobile phase. The detection wavelength was 203nm.The flow rate was 1 ml/min. Results:The linear range of ginsenoside Rg1, ginsenoside Re were both 0.4~1.6μg (r=0.9996). The average recovery of the method:ginsenoside Rg1 was 98.68%, RSD was 171%(n=9);ginsenoside Re 100.81%, 2.71%(n=9). Conclusion:the content was determined by the method of ginsenoside Rg1, ginsenoside Re in Jiangtangning capsules, the methods are accurate, high sensitivity, and can effectively control the quality of the products. |
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Keywords: | Jiangtangning capsules ginsenoside Rgl ginsenoside Re HPLC |
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