葫芦素B纳米脂质载体对结肠癌和乳腺癌细胞抑制及凋亡作用的研究 |
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引用本文: | 李瑶,马晓星,刘畅,潘虹,李津明,韩翠艳. 葫芦素B纳米脂质载体对结肠癌和乳腺癌细胞抑制及凋亡作用的研究[J]. 黑龙江医药, 2014, 0(4): 773-776 |
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作者姓名: | 李瑶 马晓星 刘畅 潘虹 李津明 韩翠艳 |
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作者单位: | 李瑶(哈尔滨商业大学药学院 黑龙江哈尔滨150028 齐齐哈尔医学院药学院 黑龙江齐齐哈尔161006); 马晓星 (齐齐哈尔医学院药学院 黑龙江齐齐哈尔161006); 刘畅 (齐齐哈尔医学院药学院 黑龙江齐齐哈尔161006); 潘虹 (齐齐哈尔医学院药学院 黑龙江齐齐哈尔161006); 李津明 (厦门医学高等专科学校 福建厦门361008); 韩翠艳 (齐齐哈尔医学院药学院 黑龙江齐齐哈尔161006); |
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基金项目: | 黑龙江省教育厅面上项目资助(项目编号:12531787) |
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摘 要: | 目的:研究葫芦素B纳米脂质载体(CuB-NLC)对结肠癌Lovo细胞,乳腺癌MCF-7细胞的体外细胞毒性。方法:结肠癌Lovo细胞,乳腺癌MCF-7细胞常规复苏、培养及传代,取处于对数生长期的细胞用于实验。采用CCK-8法检测质量浓度为0、0.0625、0.25、1.00、4.00、16.0 mg/L的CuB-NLC对结肠癌Lovo细胞,乳腺癌MCF-7细胞的毒性,流式细胞仪检测结肠癌Lovo细胞,乳腺癌MCF-7细胞的细胞周期进程及Annexin V/PI双染法检测细胞的凋亡率。结果:与葫芦素B (CuB)相比CuB-NLC对结肠癌Lovo细胞,乳腺癌MCF-7细胞生长抑制作用明显增强(P<0.05),且这种抑制作用随药物浓度增加而增强。CuB组及CuB-NLC组作用于结肠癌Lovo细胞、乳腺癌MCF-7细胞48 h后,与NLC空白组及DMSO对照组相比较,CuB及CuB-NLC均能够引起结肠癌Lovo细胞、乳腺癌MCF-7细胞G2/M期阻滞,同时伴有G0/G1期细胞减少, CuB-NLC组的作用更加明显,有统计学差异(P<0.05)。Annexin V/PI双染法检测结果表明,CuB-NLC能显著诱导结肠癌Lovo细胞,乳腺癌MCF-7细胞凋亡。结论:葫芦素B纳米脂质载体能够抑制结肠癌Lovo细胞,乳腺癌MCF-7细胞的增殖,诱导细胞凋亡。
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关 键 词: | 葫芦素B 纳米脂质载体 细胞毒性 细胞周期 凋亡 |
Inhibition and Apoptosis Induced by Cucurbitacin B Nanostructured Lipid Carriers in Colon Cancer and Breast Cancer Cells |
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Affiliation: | LI Yao , et al (School of Pharmacy, Harbin University of Commerce( Harbin 150028, China)) |
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Abstract: | Objective:To evaluate the cytotoxicity of Cucurbitacin B nanostructured lipid carrier (NLC) on colon cancer Lovo cells and breast cancer MCF-7 cells in vitro. Methods:The colon cancer Lovo cells and breast cancer MCF-7 cells were routine resuscitation, culture, subculture. Cells in logarithmic growth phase used in experiments. The colon cancer Lovo cells and breast cancer MCF-7 cells were treated with 0、0.0625、0.25、1.00、4.00、16.0 mg/L of Cucurbitacin B nanostructured lipid carrier (NLC) at various concen-trations. Growth suppression was evaluated by CCK-8 method;Flow cytometry( FCM) was used to investigate the distribution of cell life and Annexin V/PI double staining method was used to observe the early apoptosis induced by Cucurbitacin B (CuB) and Cucurbit-acin B nanostructured lipid carrier (NLC) in colon cancer Lovo cells and breast cancer MCF-7 cells. Results:Cucurbitacin B nano-structured lipid carrier (NLC) showed greater growth suppression compared with Cucurbitacin B (CuB)(P〈0.05), and the inhibition in-creased with the increase of drug concentraton. After Cucurbitacin B (CuB) and Cucurbitacin B nanostructured lipid carrier (NLC) treated colon cancer Lovo cells and breast cancer MCF-7 cells 48h, compared with Blank NLC group and DMSO control group, Cucurbitacin B (CuB) and Cucurbitacin B nanostructured lipid carrier (NLC) could make Lovo and MCF-7 cells G2/M period increasing, G0/G1 phase reducing at the same time. The effect of Cucurbitacin B nanostructured lipid carrier (NLC) is more obvious(P〈0.05). An-nexin V/PI double staining method test indicated that Cucurbitacin B nanostructured lipid carrier (NLC) could induce Lovo and MCF-7 cells. Conclusion:Cucurbitacin B nanostructured lipid carrier (NLC) could inhibit colon cancer Lovo cells and breast cancer MCF-7 cells in vitro. This inhibitory effect may be induce colon cancer Lovo cells and breast cancer MCF-7 cells apoptosis. |
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Keywords: | cytotoxicity of cucurbitacin B nanostructured lipid carrier cytotoxicity cell life apoptosis |
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