葫芦素B纳米脂质载体对结肠癌和乳腺癌细胞抑制及凋亡作用的研究

目的：研究葫芦素B纳米脂质载体（CuB-NLC）对结肠癌Lovo细胞，乳腺癌MCF-7细胞的体外细胞毒性。方法：结肠癌Lovo细胞，乳腺癌MCF-7细胞常规复苏、培养及传代，取处于对数生长期的细胞用于实验。采用CCK-8法检测质量浓度为0、0.0625、0.25、1.00、4.00、16.0 mg/L的CuB-NLC对结肠癌Lovo细胞，乳腺癌MCF-7细胞的毒性，流式细胞仪检测结肠癌Lovo细胞，乳腺癌MCF-7细胞的细胞周期进程及Annexin V/PI双染法检测细胞的凋亡率。结果：与葫芦素B （CuB）相比CuB-NLC对结肠癌Lovo细胞，乳腺癌MCF-7细胞生长抑制作用明显增强（P＜0.05），且这种抑制作用随药物浓度增加而增强。CuB组及CuB-NLC组作用于结肠癌Lovo细胞、乳腺癌MCF-7细胞48 h后，与NLC空白组及DMSO对照组相比较，CuB及CuB-NLC均能够引起结肠癌Lovo细胞、乳腺癌MCF-7细胞G2/M期阻滞，同时伴有G0/G1期细胞减少， CuB-NLC组的作用更加明显，有统计学差异（P＜0.05）。Annexin V/PI双染法检测结果表明，CuB-NLC能显著诱导结肠癌Lovo细胞，乳腺癌MCF-7细胞凋亡。结论：葫芦素B纳米脂质载体能够抑制结肠癌Lovo细胞，乳腺癌MCF-7细胞的增殖，诱导细胞凋亡。

Inhibition and Apoptosis Induced by Cucurbitacin B Nanostructured Lipid Carriers in Colon Cancer and Breast Cancer Cells

Objective：To evaluate the cytotoxicity of Cucurbitacin B nanostructured lipid carrier （NLC） on colon cancer Lovo cells and breast cancer MCF-7 cells in vitro. Methods：The colon cancer Lovo cells and breast cancer MCF-7 cells were routine resuscitation, culture, subculture. Cells in logarithmic growth phase used in experiments. The colon cancer Lovo cells and breast cancer MCF-7 cells were treated with 0、0.0625、0.25、1.00、4.00、16.0 mg/L of Cucurbitacin B nanostructured lipid carrier （NLC） at various concen-trations. Growth suppression was evaluated by CCK-8 method;Flow cytometry（ FCM） was used to investigate the distribution of cell life and Annexin V/PI double staining method was used to observe the early apoptosis induced by Cucurbitacin B （CuB） and Cucurbit-acin B nanostructured lipid carrier （NLC） in colon cancer Lovo cells and breast cancer MCF-7 cells. Results：Cucurbitacin B nano-structured lipid carrier （NLC） showed greater growth suppression compared with Cucurbitacin B （CuB）（P〈0.05）, and the inhibition in-creased with the increase of drug concentraton. After Cucurbitacin B （CuB） and Cucurbitacin B nanostructured lipid carrier （NLC） treated colon cancer Lovo cells and breast cancer MCF-7 cells 48h, compared with Blank NLC group and DMSO control group, Cucurbitacin B （CuB） and Cucurbitacin B nanostructured lipid carrier （NLC） could make Lovo and MCF-7 cells G2/M period increasing, G0/G1 phase reducing at the same time. The effect of Cucurbitacin B nanostructured lipid carrier （NLC） is more obvious（P〈0.05）. An-nexin V/PI double staining method test indicated that Cucurbitacin B nanostructured lipid carrier （NLC） could induce Lovo and MCF-7 cells. Conclusion：Cucurbitacin B nanostructured lipid carrier （NLC） could inhibit colon cancer Lovo cells and breast cancer MCF-7 cells in vitro. This inhibitory effect may be induce colon cancer Lovo cells and breast cancer MCF-7 cells apoptosis.