| 重组人内皮抑素诱导人脐带静脉血管内皮细胞凋亡 |
| |
| 引用本文： | 杨芳,何援利.重组人内皮抑素诱导人脐带静脉血管内皮细胞凋亡[J].南方医科大学学报,2006,26(7):978-980. |
| |
| 作者姓名： | 杨芳 何援利 |
| |
| 作者单位： | 南方医科大学珠江医院妇产科,广东,广州,510282 |
| |
| 摘 要： | 目的观察人内皮抑素重组蛋白对人脐带静脉血管内皮细胞增殖的影响。方法采用细胞凋亡的荧光检测法检测对内皮细胞凋亡的影响。MTT法观察不同浓度的重组人内皮抑素对人脐带静脉血管内皮细胞（ECV304）增殖的抑制作用。结果重组人内皮抑素具有明显的抑制内皮细胞增殖的作用，ED50=550ng／ml，随重组蛋白浓度的增加抑制作用更为明显。结论重组人内皮抑素对细胞增殖的抑制与其浓度呈正相关，存在剂量效应关系；内皮抑素抗血管生成的机制与其抑制内皮细胞增殖、诱导内皮细胞凋亡有关。
|
| 关 键 词： | 人内皮抑素 内皮细胞 细胞凋亡 |
| 文章编号： | 1673-4254（2006）07-0978-03 |
| 收稿时间： | 2005-12-10 |
| 修稿时间： | 2005年12月10 |
Recombinant human endostatin induces apoptosis of human umbilical venous endothelial cells |
| |
| YANG Fang,HE Yuan-li.Recombinant human endostatin induces apoptosis of human umbilical venous endothelial cells[J].Journal of Southern Medical University,2006,26(7):978-980. |
| |
| Authors: | YANG Fang HE Yuan-li |
| |
| Institution: | Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. |
| |
| Abstract: | To investigate the effects of human endostatin on proliferation of human umbilical venous endothelial cells, the apoptosis of the endothelial ceils treated with recombinant human endostatin was examined by fluorescence method and the cell proliferation activity analyzed by MTT assay. The results showed that human endosmtin inhibited the proliferation of the endothelial ceils with ED~0 of 550 ng/ml, and the inhibitory effect was enhanced with the increase of endostatin concentration, suggesting a dose-dependent inhibitory effect of human endostatin on endothelial cell proliferation. The mechanism of antiangiogenesis induced by human endostatin is related to the inhibition of cell proliferation and induction of apoptosis of the endothelial ceils. |
| |
| Keywords: | human endostatin endothelial cells apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
