心肌肥大大鼠心肌细胞凋亡与Bcl-2、Bax蛋白表达的变化

目的:探讨心肌肥大大鼠心肌细胞凋亡及Bcl-2和Bax蛋白表达的变化。方法:腹腔注射异丙肾上腺素,建立大鼠心肌肥大模型。采用原位末端缺口标记(TUNEL)法观察心肌细胞凋亡;应用Westernblotting和免疫组化方法检测Bcl-2和Bax的蛋白表达,并使用HPIAS10009图像分析仪进行定量分析。结果:与对照组比较,心肌肥大组心肌细胞凋亡显著增加(P<0.01),Bax表达增加(P<0.01),而Bcl-2表达减少(P<0.01),Bcl-2/Bax比值下降(P<0.01)。结论:Bcl-2和Bax基因参与了心肌肥大心肌细胞凋亡的调控。

Cardiomyocyte apoptosis and the expression of Bcl-2 and Bax protein in rats with myocardial hypertrophy

Objective： To research the change of cardiomyocyte apoptosis and the expression of Bcl- 2 and Bax protein in rats with myocardial hypertrophy. Methods： Myocardial hypertrophy model was established by injecting isoprenaline in hypodermic of rats. Terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling （TUNEL） was used to detect myoeardium apoptosis. Western blot and immunohistocheminstry wrer used respectively to assay the protein expression of Bel-2 and Bax in myocardial tissues,and quantified HPIAS10009 computerized image analytical instrument. Results： Compared with control group, eardiomyocyte apoptotic index increased （P〈0.01） , expression of Bax increased （P〈0.01）, whereas expression of Bcl-2 （P（0.01） and the radio of Bel-2/ Bax decreased （P〈0. 01） in myocardial hypertrophy group. Conclusion： Cardiomyocyte apoptosis is regulated by Bcl-2 and Bax gene in myocardial hypertrophy.