重症肌无力患者造血干/祖细胞及其亚群富集纯化的研究 |
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引用本文: | 秦超,莫雪安,陆锐,凌莉,莫武宁,孙圣刚,童萼塘.重症肌无力患者造血干/祖细胞及其亚群富集纯化的研究[J].脑与神经疾病杂志,2004,12(2):97-98. |
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作者姓名: | 秦超 莫雪安 陆锐 凌莉 莫武宁 孙圣刚 童萼塘 |
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作者单位: | 1. 430022,华中科技大学同济医学院协和医院神经内科,武汉 2. 广西医科大学第一附属医院 |
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基金项目: | 广西科技攻关项目资助(桂科攻0322025-9) |
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摘 要: | 目的:获得高纯度造血干/祖细胞及其亚群,用于重症肌无力(MG)造血干细胞移植治疗和生物学特性的研究。方法:用免疫磁珠法(MiniMACS)从MG骨髓中富集CD34+细胞后再用荧光激活细胞分选(Fluorescent activatedcell sorter,FACS)纯化CD34+/CD38+和CD34+/CD38-细胞亚群。结果:MiniMACS分选的CD34+细胞群纯度达90%,FACS分选的CD34+/CD38-和CD34+/CD38+两细胞亚群纯度可达95%以上。结论:联合使用MiniMACS和FACS可迅速大量纯化HSC/HPC及其重要细胞亚群,可用于临床造血于细胞移植、基因治疗和研究HSC/HPC及其亚群生物学特性。
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关 键 词: | 造血干/祖细胞 免疫磁珠法 流式细胞计量术 重症肌无力 |
文章编号: | 1006-351X(2004)02-0097-02 |
修稿时间: | 2003年11月4日 |
Collection and purification of myasthenia gravis CD34 + hematopoietic stem(progenitor) cells and its sup-groups |
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Abstract: | Objective: To collect and purify the hone marrow CD34+ hematopoietic stem cells and its sub-groups from the patients with myasthenia gravis. Methods; CD34+ cells were collected aand purified by means of imunomagnetic beads on MiniMACS device. Flow cytometry was employed to determine percentage of CD34+ cells, CD34+/CD38- cells and CD34+/CD38+ cells before and after miniMACS. and to detect cell ability after CD34+ cell purification. Results: The purity of CD34+ cells amounted to more than 90 % after the pruification, The purity of CD34+/CD38- cells and CD34+/CD38+ cells amounted to more than 95% after FACS. Conclusions: The Conbination of MiniMACS and FACS can sort effectively the bone marrow CD34+ cells and its sub-groups so that the bone marrow CD34+ cells and its sub-groups are used for hematopoietic stem cell transplantation, gene therapy and for study of HSC/HPC and its cell sub-groups. |
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