血管活性肠肽对大鼠Leydig细胞的免疫调节作用

目的:研究血管活性肠肽(VIP)对大鼠睾丸间质细胞(Leydig细胞)免疫功能的调节。方法:以VIP作用于溶脲脲原体(UU)感染的SD大鼠及大鼠Leydig细胞为研究对象,观察VIP对大鼠Leydig细胞分泌的细胞因子(IL-1、IL-6及TGF-β)和FasL表达的调节,从体外及动物整体水平来探讨VIP对大鼠Leydig细胞免疫功能的调节。结果:在睾丸局部感染时,VIP能调节Leydig细胞IL-1、IL-6、TGF-β及FasL的分泌和表达格局;同时VIP对大鼠睾丸局部免疫豁免的调节和维持也起到一定的作用。结论:VIP能调节大鼠Leydig细胞的免疫功能,并参与调节大鼠睾丸局部的免疫豁免。

Immunoregulation of vasoactive intestinal peptide on rat Leydig cells

AIM: To study the regulation of immune function of rat Leydig cells by vasoactive intestinal peptide (VIP). METHODS: Rat Leydig cells were seperated and infected by UU, with or without the incubation with VIP. The expression of FasL on Leydig cells in different group was analysed by flow cytometry(FCM). The mRNA expression of IL-1, IL-6, TGF-beta and FasL of Leydig cells in different group was identified by RT-PCR. SD rats were infected by UU with or without the injection of VIP, and the testis tissnes of each group were har vested and observed under transmission electron microscope. RESULTS: When testis was infected by UU, VIP up-regulated the mRNA expression of IL-1, IL-6, and TGF-beta, and down-regulated the expression of FasL in vitro. In addition, there was significant difference in testis morphous of rats from different group. CONCLUSION: VIP could regulate the expression pattern of IL-1, IL-6, TGF-beta and FasL by Leydig cells, which may contribute to maintain immune privilege of the testis. VIP could regulate rat Leydig cell immune function.