中国汉族人群hGSTA1基因C-69T多态性分析

目的　研究中国汉族人群中人谷胱甘肽S 转移酶(hGSTA1 )C-69T基因多态性的分布。方法1 4 0例血样本来自中国2 5个省份的汉族人口,用聚合酶链反应 限制性片段长度多态性方法检测hGSTA1 69位点的变异。结果　中国汉族人群GSTA1基因 69位点的野生型纯合子(CC)基因型的分布频率为75 .0 %,突变型纯合子(TT)基因型为0 .7%,杂合子(CT)基因型为2 4 .3 %;C及T两种等位基因的频率分别为87.1 %及1 2 .9%。结论中国汉族人群GSTA1基因呈多态性分布,其等位基因和基因型频率不同于其他种族

Genetic polymorphism of human glutathione S-transferase A1 in population of Han nationality in China

AIM To investigate the distribution of glutathione S-transferase A1(GSTA1) genetic polymorphism at locus -69 in population of Han nationality in China. METHODS Genomic DNA was extracted from human peripheral blood and the genotypes of GSTA 1 gene polymorphism were analyzed by polymerase chain reaction-restricted fragment length polymorphism(PCR-RFLP) in 140 Chinese Han people. RESULTS(In the population), the proportion of CC, CT and TT genotypes were 75.0%, 24.3% and 0.7%, respectively. Allele frequencies of C and T were detected to be 87.1% and 12.9%, respectively. CONCLUSION The distribution of GSTA1 genes is polymorphism in Chinese Han population and different from those in other racial populations.