头颈部鳞癌端粒酶活性定性检测

目的 :研究原发头颈部鳞癌及相应癌旁组织中端粒酶活性表达 ,并探讨其作为头颈部鳞癌分子生物学标志物的可能性。方法 :采用TRAP银染法 ,对 32例原发头颈部鳞癌及 15例癌旁组织进行端粒酶活性检测。结果 :32例原发头颈部鳞癌端粒酶阳性率为 84 .4 % (2 7 32 ) ,15例癌旁组织阳性率为 33.3% (5 15 )。有淋巴结累及(86 .7% )和无淋巴结累及 (82 .4 % )以及低分化鳞癌 (90 % )和中、高分化鳞癌 (81.8% )端粒酶阳性率无统计学差异。端粒酶活性与肿瘤原发部位、临床分期无相关性。结论 :端粒酶活化与头颈部鳞癌的发生发展有密切关系 ,可能是癌变的早期事件

Telomerase Activity in Human Head and Neck Squamous Cell Carcinoma and Adjacent Tissues

Objective: To investigate the telomerase activity in human head and neck squamous cell carcinomas and their adjacent tissues and to explore the possibility of telomerase activity as tumor biological marker. Methods: Thirty two patient samples of primary head and neck squamous cell carcinoma and 15 adjacent tissues were assayed using TRAP Silver staining for detection of telomerase activity. Results: Twenty seven of 32 (84.4%) cancer samples and 5 of 15 (33.3%) adjacent samples were telomerase positive (P<0.05, Chi Square Test). The frequency of telomerase activation was lower in cancer without lymph node involvement (86.7%) than that with lymph node involvement (82.4%), but the difference is no statistical significance. No obvious correlation was found between the telomerase activity and the other clinical parameters such as primary site and staging. Conclusion: The finding of telomerase activity in 84.4% of primary head and neck squamous cell carcinoma samples is consistent with concept of telomerase playing a key role in tumorigenesis; telomerase activation may be the early event in tumorigenesis.