| Effects of Phenamiphos on Muscarinic Acetylcholine Receptor in the Brain Synaptosome in Rats and Its Mechanism |
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| 引用本文： | 孙黎,马谭庚,刘毓谷. Effects of Phenamiphos on Muscarinic Acetylcholine Receptor in the Brain Synaptosome in Rats and Its Mechanism[J]. 华中科技大学学报(医学英德文版), 1994, 14(2): 89-93. DOI: 10.1007/BF02886782 |
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| 作者姓名： | 孙黎 马谭庚 刘毓谷 |
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| 作者单位： | Department of Environmental Toxicology，Tongji Medical University，Wuhan |
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| 摘 要： | ＥｆｆｅｃｔｓｏｆＰｈｅｎａｍｉｐｈｏｓｏｎＭｕｓｃａｒｉｎｉｃＡｃｅｔｙｌｃｈｏｌｉｎｅＲｅｃｅｐｔｏｒｉｎｔｈｅＢｒａｉｎＳｙｎａｐｔｏｓｏｍｅｉｎＲａｔｓａｎｄ Ｉｔｓ ＭｅｃｈａｎｉｓｍＳＵＮＬｉ（孙黎），ＭＡＴａｎ－ｇｅｎｇ（马谭庚），ＬＩ...
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| 收稿时间： | 1994-01-20 |
Effects of phenamiphos on muscarinic acetylcholine receptor in the brain synaptosome in rats and its mechanism |
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| Sun Li,Ma Tan-geng,Liu Yu-gu. Effects of phenamiphos on muscarinic acetylcholine receptor in the brain synaptosome in rats and its mechanism[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 1994, 14(2): 89-93. DOI: 10.1007/BF02886782 |
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| Authors: | Sun Li Ma Tan-geng Liu Yu-gu |
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| Affiliation: | (1) Department of Environmental Toxicology, Tongji Medical University, Wuhan |
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| Abstract: | Summary The studies were performed to investigate the mechanism of phenamiphos affecting muscarinic acetylcholine receptor (M-AchR) of the brain synaptosome in rats. The results showed that phenamiphos inhibited the binding of3H-quinuclidinyl benzilate (3 H-QNB) to the brain synaptosome in rats, and this effect was dose and time-dependent. This suggests that phenamiphos might directly affect M-AchR. The KD and Bmax of phenamiphos to M-AchR were measured and found to be 1. 3×10-7 mol/L and 4. 57 pmol/mg protein, respectively. It belongs to non-specific inhibition of phenamiphos on the binding of3H-QNB to M-AchR. It was also found that phenamiphos decreased the level of protein sulfhydryl group in brain synaptosome membrane. Dithiothreitol (DTT) and the reduced glutathion (GSH) prevented the decrease of protein sulfhydryl group and the inhibition of phenamiphos on the binding of3H-QNB to M-AchR. These showed that phenamiphos could affect the sulfhydryl group of M-AchR surface. |
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| Keywords: | organophosporous insecticides acetylcholine receptor sulfhydryl group |
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