| HPLC-ELSD法测定不同来源黄芪药材中黄芪甲苷含量 |
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| 引用本文： | 刘杨,;包华音,;单成钢. HPLC-ELSD法测定不同来源黄芪药材中黄芪甲苷含量[J]. 山东中医杂志, 2014, 0(12): 1019-1021 |
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| 作者姓名： | 刘杨, 包华音, 单成钢 |
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| 作者单位： | [1]山东中医药大学,山东济南250355; [2]山东省农业科学院,山东济南250100 |
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| 基金项目： | 山东省农业良种工程重大课题（编号：2011LZ01-03） |
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| 摘 要： | 目的:测定不同来源黄芪药材中黄芪甲苷的含量,为黄芪药材的质量评价提供实验依据。方法:采用HPLC-ELSD法,色谱条件:Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-水(32∶68);流速:1.0 m L·min-1;柱温:30℃。ELSD参数:载气流速2.7 L·min-1;漂移管温度:105℃。结果:黄芪甲苷的线性范围为2.5~12.5μg,r=0.9996(n=6),平均加样回收率为102.40%(RSD1.76%)。结论 :不同产地黄芪药材黄芪甲苷含量存在较大差异。该方法简便快捷,重现性好,可广泛用于黄芪药材的质量控制与评价。
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| 关 键 词： | 黄芪 黄芪甲苷 HPLC-ELSD |
Determination of Astragaloside IV in Astragali Radix from Different Areas by HPLC-ELSD |
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| Affiliation: | LIU Yang,BAO Huayin,SHAN Chenggang (1.Shandong University of Traditional Chinese Medicine,Jinan 250355,China; 2.Shandong Academy of Agri- cultural Sciences, Jinan 250100, China) |
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| Abstract: | Objective：To determine astragaloside 1V in Astragali Radix from different areas by HPLC-ELSD so as to provide experimental basis for quality evaluation of Astragali Radix. Method：HPLC-ELSD was used in the quantitative analysis of astragaloside IV. The measurement conditions were as follows：Diamonsil ClS （250mm×4.6 mm,5μm） column,and mobile phase：acetonitrile-water （32：68）,and flow rate：l.0 mL-min-l,and column temperature ： 30 ℃, and detector： ELSD 2000, and gas flow： 2.7 L. min-1, and the temperature of drift tube ： 105 ℃. Results：The linear range of astragaloside IV was 2.5 μg-12.5 μg,r=0.9996 （n=6）,the average recovery was 102.40%（RSD1.76%）. Conclusion：There were great differences in astragaloside IV content of Astragali Radix from different areas. The method is of simplicity,accuracy and good reproducibility ,and can be widely used in the quality control and evaluation of A stragali Radix. |
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| Keywords: | A stragali Radix astragaloside Ⅳ HPLC-ELSD |
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