GC/MS analysis of phencyclidine acid metabolite in human urine |
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Authors: | M A elSohly T L Little J M Mitchell B D Paul L D Mell J Irving |
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Affiliation: | ElSohly Laboratories, Oxford, Mississippi 38655. |
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Abstract: | Available methods for determining PCP use are based on the presence of the parent drug in urine. PCP, however, is very potent and is extensively metabolized; it is therefore present in urine in only small quantities. This work was undertaken to determine whether an amino acid metabolite of PCP, 5-(N-(1'-phenylcyclohexyl)amino)pentanoic acid, can be used to determine PCP use. A solid phase adsorption technique was developed to extract the amino acid metabolite from urine. Recovery averaged 93%, and subsequent GC/MS analysis was free from interference. Analysis of 67 urine samples demonstrated that the amino acid metabolite exists in human urine in significant quantities. |
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