X射线全身照射诱导小鼠派伊氏板细胞凋亡及其机理的研究

目的 研究不同剂量X射线全身照射后小鼠派伊氏板细胞凋亡相关基因蛋白表达的变化规律。初步阐明凋亡调控的部分分子机理。方法 应用光、电镜技术观察小鼠派伊氏板细胞凋亡的形态改变，并用流式细胞术检测小鼠派伊氏板细胞Bcl-xL及Fas-L蛋白表达的变化。结果 2GyX射线全身照射后派伊氏板细胞凋亡增加，且Bcl-xL蛋白表达下降，Fas-L蛋白表达升高；75mGyX射线全身照射后，派伊氏板细胞凋亡减少，且Bcl-xL蛋白表达升高，Fas-L蛋白表达下降。结论 凋亡相关基因Bcl-xL、Fas-L在电离辐射诱导的派伊氏板细胞凋亡方面起重要的调控作用。

Studies on induction of apoptosis in mouse Peyer's patches by whole body X-irradiation and its mechanisms

Objective\ To study the effect of apoptosis\|related genes in mouse Peyer's patches and its molecular mechanism after whole body irradiation(WBI) with different doses of X\|rays. Methods\ Light microscopy and transmission electron microscopy were used to observe the morphology and structure of Peyer's patches and flow cytometry (FCM) was used to detect the changes of Bcl\|x\-L and Fas\|L proteins. Results\ The apoptosis in Peyer's patches was increased after 2 Gy X\|rays and decreased after 75 mGy X\|rays.The expression of Bcl\|x\-L protein was decreased and the expression of Fas\|L protein was increased in Peyer's patches after WBI with 2 Gy X\|rays,whereas the former increased and the latter decreased after WBI with 75 mGy. Conclusion\ Apoptosis\|related genes such as Bcl\|x\-L,Fas\|L might play an important role in regulation of radiation\|induced apoptosis in Peyer's patches.