| 激光照射对弓形虫速殖子作用的流式细胞分析 |
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| 引用本文： | 陆绍红,林爱芬,吕庆华,陈睿. 激光照射对弓形虫速殖子作用的流式细胞分析[J]. 中国病原生物学杂志, 2000, 13(3): 175-177 |
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| 作者姓名： | 陆绍红 林爱芬 吕庆华 陈睿 |
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| 作者单位： | 1. 浙江省医学科学院寄生虫病研究所,浙江杭州,310013
2. 浙江大学医学院附属第二医院肿瘤研究所 |
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| 摘 要： | 用流式细胞术 (FCM)检测分析了激光照射对弓形虫 RH株速殖子的作用。结果显示 ,激光照射后弓形虫速殖子群体 DNA含量分布发生改变 ,DNA含量的第 1道数峰值 (平均值 )左移 ,第 1峰值分布范围内速殖子数占总速殖子数百分率增加 ,3.5 W和 4.5 W剂量照射组与对照组相比差异存在显著性 (P<0 .0 5 )。提示激光照射能抑制弓形虫 DNA的合成 ,减少弓形虫群体 DNA的含量
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| 关 键 词： | 激光照射 弓形虫 速殖子 流式细胞仪 DNA含量 |
| 修稿时间： | 1999-05-07 |
FLOW CYTOMETYIC ANALYSIS OF LASER-IRRADIATED ON TOXOPLASMA GONDII TACHYZOITES |
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| LU Shao-hong,LIN Ai-fen,LU Qing-hua,CHEN Rui. FLOW CYTOMETYIC ANALYSIS OF LASER-IRRADIATED ON TOXOPLASMA GONDII TACHYZOITES[J]. Journal of Pathogen Biology, 2000, 13(3): 175-177 |
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| Authors: | LU Shao-hong LIN Ai-fen LU Qing-hua CHEN Rui |
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| Abstract: | The effect of laser irradiated on Toxoplasma gondii tachyzoites(RH strain) population(10 4 organisms) was analyzed by flow cytometry. The results showed that the DNA content of laser irradiated Toxoplasma changed. The peak value(mean value) of the first peak of DNA content decreased from 100 to 60, and the percentage of tachyzoite number in the first peak increased, especially 3.5 W and 4.5 W laser irradiated groups had great difference comparing with control group( P <0.05). Thus we deem that laser irradiation can inhibit the DNA synthesis, and decrease the DNA content of T. gondii tachyzoites. |
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| Keywords: | Laser irradiation Toxoplasma gondii tachyzoites flow cytometry DNA content |
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