重组腺病毒载体转染p16基因对肝癌细胞的生长抑制作用

目的：通过腺病毒转染p16基因观察对肝癌SMMC-7721细胞的杀伤作用。方法：克隆人p16基因全长cDNA序列，构建携带p16基因的增殖缺陷型重组腺病毒p16(AdV-p16)，以AdV-p16感染人肝癌SMMC-7721细胞，观察p16基因的表达及其对细胞的生长抑制或杀伤作用。结果：AdV．p16能介导外源基因p16在肝癌SMMC-7721细胞系中高效表达。在感染AdV-p16后随重复感染度(MOI)升高p16基因阳性表达率升高，四甲基偶氮唑蓝染色法(MTT)实验结果表明，SMMC-7721细胞的生长受抑制。结论：腺病毒能够介导p16基因在肝癌细胞中高效表达，促进细胞周期阻滞和诱导肿瘤细胞凋亡。

Inhibitory Effects of p16 Gene Mediated by Recombinant Adenovirus on Hepatocellular Carcinoma Cell Line

To study the inhibitory effect of p16 gene on hepatocellular carcinoma cell line by transfection recombinant adenovirus. Methods: To construct the recombinant adenovirus vector AdV-p16 carrying p16 gene, and to transduce AdV-p16 into human hepatocellular carcinoma cell line SMMC-7721. The expression of p16 gene was detected by immunohistochemistry, and inhibitory effects of p16 gene on SMMC-7721 cell viability were analyzed by monotetrazolium (MTT) colorimetric assay. Results: Recombinant adenovirus AdV-p16 could infect hepatocellular carcinoma SMMC-7721 cells. The expression rate of p16 gene increased with multiplicity of infection (MOI) after the cells were transfectted with AdV-p16 vector. Pathologic observation showed that many cancer cells were died of apoptosis when infected the recombinant adenovirus AdV-p16. The experiment of MTT assay demonstrated the growth inhibitory effect of AdV-pl6 transfection on SMMC-7721 cells. Conclusion: These results suggest that p16 gene can introduce apoptosisand growth inhibition of hepatocellular carcinoma cell line. The p16 gene therapy mediated by recombinant adenovirus can induce high levels of p16 expression in carcinoma cells, and is a promising strategy for cancer treatment.