尿激酶对弱精症患者精子活力影响的研究

对２８例弱精症男子精液中加入不同浓度的尿激酶后，分别在３０、６０、９０和１２０分钟观察精子的活率和前向运动能力。并对１４例患者体内给予尿激酶１０天后，比较用药前后精子活率和有前向运动力精子数目的变化。结果：２８例精液标本体外加入尿激酶后，３０、６０、９０和１２０分钟时精子的活率均有提高；前向运动精子的数目亦有增加。体内给药前，１４例精子平均活率为４４．６４±１１．７９，用药１０天后，精子平均活率为６４．７１±１３．０１，二者比较有极显著差异（Ｐ＜０．０１）。尿激酶的这一作用可能与它降低精液的粘稠度有关。

Effect of urokinase on sperm viability and sperm motility of infertile men with astheno spermia

bjectives :To observe the effect of urokinase on sperm viability and forward motility semen of as-thenospermic patients.Design :After liquefaction of 24 normal semen samples,the urokinase of different dosage was added intothe samples Sperm viability,and then forward motility of sperm were determined after 30,60,90,120 min-utes.At the same time changes of sperm viability and numbers of forward progressive spermatozoa from 14infertile men with asthenospermia were analysed after ten days treatment with urokinase.Setting :Clinic of Department of Infertility in Center of Reproductive Medcine,Tongji MedicalUniversity.Methods:Semens were colleted by masturbation.Sperm parameters were determined according to WHOstandard manual. After liquefaction each semen sample was divided into five tubes.Except for one tubeurokinase was added to the other 4.14 infertile men with asthenospermia were treated with urokinase 10000IU/d for 10 days.Main outcome measure :Percentage of sperm viability and sperm motility.Results :During 30 and 60 minutes urokinase of 1600 IU/ml and 3 200 IU/ml apparently increased sperm viability and sperm motility, but after 90 and 120 minutes the effect of urokinase on sperm viability and sperm motility was far less than that of urokinase of 400 IU/ml and 800 IU/ml.On the other hand,the urokinase can increase percentage of sperm viability and sperm motility in vivo.Conclusion :The study indicated the effects of urokinase on sperm viability and forward progression of sperm were related to low semen viscosity caused by urokina se.The study confirmed urokinase increased sperm viability and sperm motility of asthenospermi.