| 提高免疫酶技术检测HFRS病毒抗原敏感性的初步研究 |
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| 引用本文： | 甄荣芬,薛采芳,安献禄,张荣庆,程云. 提高免疫酶技术检测HFRS病毒抗原敏感性的初步研究[J]. 医学争鸣, 1988, 0(4) |
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| 作者姓名： | 甄荣芬 薛采芳 安献禄 张荣庆 程云 |
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| 作者单位： | 第四军医大学微生物学教研室(甄荣芬，薛采芳，安献禄，张荣庆)，第四军医大学微生物学教研室(程云) |
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| 摘 要： | 以磷苯二胺为辣根过氧化物酶底物显色剂的ELISA双抗体夹心法检测HFRSV抗原中,用四甲基联苯胺替代邻苯二胺,用生物素-抗生素蛋白的BA-ELISA和高效价抗HFRSV的McAb进行McAb-ELISA,使检测抗原的敏感性分别提高4倍以上。如果四甲基联苯胺与BA-ELISA或与McAb-ELISA联合应用,则比原ELISA敏感16倍以上。
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| 关 键 词： | 流行性出血热病毒 抗原 四甲基联苯胺 单克隆抗体 生物素—亲和素系统 免疫酶技术 酶联免疫吸附试验 |
Preliminary Studies on Raising Detection Sensitivities of HFRS Virus Antigens by Improving Enzyme Immunoassay |
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| Zhen Rongfen,et al.. Preliminary Studies on Raising Detection Sensitivities of HFRS Virus Antigens by Improving Enzyme Immunoassay[J]. Negative, 1988, 0(4) |
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| Authors: | Zhen Rongfen et al. |
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| Affiliation: | Zhen Rongfen,et al. Department of Microbiology |
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| Abstract: | The EL1SA methods described in this report was based on double antibody ELISA sandwich method to detect antigens of HFRS virus in which o-PD was used as substract for HRP. In these methods, when TMB was substituted for o-PD, or biotin-avidin ELISA (BA-EL1SA) was applied, or high titre McAbs against HFRSV were used (Me Ab-ELlSA), the sensitivities of detection of antigens increased over 4 times, respectively. If both TMB and BA-EL1SA or both TMB and McAb-ELlSA were used,the sensitivities were over 16 times higher than the original method. |
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| Keywords: | epidemic hemorrhagic fever viruss tetramethyl-benzidine monoclonal antibody biotin-avidin system enzyme immunoassay enzyme-linked immunosor-bent assay |
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