A rapid method for isolation of piperine from the fruits of <Emphasis Type="Italic">Piper nigrum</Emphasis> Linn. |
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Authors: | Niranjan Kanaki Mansi Dave Harish Padh Mandapati Rajani |
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Institution: | (1) Department of Phytochemistry and Pharmacognosy, B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Thaltej-Gandhinagar highway, Thaltej, Ahmedabad, 380 054, Gujarat, India |
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Abstract: | A simple, rapid and efficient method has been developed for the isolation of piperine from the fruits of Piper nigrum. The method involves extraction of the fruit powder with glacial acetic acid, from which piperine is partitioned into chloroform
and subsequently crystallized. The identity of the compound was confirmed by its melting point, comparison of UV, IR, and
mass spectral data with those from a reference standard, and co-chromatography with the reference standard using thin-layer
chromatography (TLC). The purity of the compound was ascertained by TLC, by recording UV absorption spectra at the start,
middle, and end positions of the spot on the plate, and by differential scanning calorimetry (DSC). |
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Keywords: | Piper nigrum Piperine |
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