舒肝凉血方联合三苯氧胺对雌激素依赖性乳腺癌的抑瘤作用

目的:研究中药舒肝凉血方联合三苯氧胺(TAM)对荷雌激素依赖性乳腺癌裸鼠的肿瘤抑制作用,并探讨其可能的机制。方法:建立裸鼠MCF-7乳腺癌移植瘤,随机分为对照组、TAM组、舒肝凉血汤组(简称中药)及中药+TAM组,给药28d后,计算肿瘤抑制率,采用流式细胞技术观察肿瘤细胞周期分布情况,采用TUNEL法观察肿瘤细胞凋亡情况,采用放射免疫法检测裸鼠血清中的雌二醇和孕酮水平。结果:治疗各组移植瘤生长速度均低于对照组,TAM组、中药组及中药+TAM组的抑瘤率分别为28.30%、26.30%、41.01%,各治疗组的平均瘤重与对照组相比差异有统计学意义(P<0.01);治疗各组G0/G1期细胞增多,出现G0/G1期细胞阻滞,其中中药+TAM组周期阻滞最明显;凋亡检测发现中药+TAM组的凋亡率显著高于对照组(P<0.001),且高于单独应用中药和TAM组;中药组和中药+TAM组的血清雌二醇水平显著低于对照组(P<0.0001)。结论:舒肝凉血方与TAM联合应用,可以使TAM的抑瘤作用增强,此作用可能与中药复方诱导细胞周期阻滞和细胞凋亡,以及降低动物血清中雌二醇水平有关。

Anti-tumor Effect of Shu-Gan-Liang-Xue Decoction Combined with Tamoxifen on Estrogen-dependent Breast Cancer

Objective:To investigate the anti-tumor effect of Shu-Gan-Liang-Xue(SGLX) decoction from traditional Chinese midicine(TCM) combined with tamoxifen on the graft model of human breast cancer in nude mice and also to analyze the possible mechanism.Methods:The animal model of MCF-7 implanted tumor in nude mice was copied.Mice with implanted tumor were randomly divided into control,tamoxifen(TAM),SGLX and SGLX+TAM groups.After SGLX decoction was given to mice for 28 days,the inhibitory rate of tumor weight was determined.Cell cycle and cell apoptosis of breast cancer were detected with flow cytometry and TUNEL respectively.The serum estradiol and progesterone were tested by radio-immunity.Results:The inhibitory rate of tumor-weight was 28.30%,26.30%,41.01% respectively in TAM,SGLX and SGLX+TAM groups,with significant difference compared with the control(P<0.01).Tumor cells in TAM,SGLX and SGLX+TAM groups were blocked at G₀/G₁ phase.The apoptotic rate was increased obviously in SGLX+TAM group.The serum estradiol was lower in SGLX and SGLX+TAM groups compared with the control at significant level(P< 0.000 1).Conclusions:SGLX decoction can increase the inhibitory effect of TAM on breast cancer synergetically.The possible mechanism include that SGLX may arrest tumor cells in G₀/G₁ phase,induce tumor cell apoptosis,and reduce serum estradiol level.