重症烧伤大鼠早期外周血单个核细胞凋亡的实验研究

目的：探讨烧伤后早期外周血单个核细胞（ＰＢＭＣ）凋亡的发生、变化规律及ｂｃｌ－２蛋白表达的变化。方法：应用流式细胞仪、ＤＮＡ凝胶电泳等手段观察烧伤大鼠ＰＢＭＣ凋亡发生的动力学变化、ｂｃｌ－２蛋白的表达及烧伤血清对细胞凋亡的影响。结果：烧伤后６ｈ起，ＰＢＭＣ凋亡率明显增加，随时间的推延呈增高趋势，此时ｂｃｌ－２蛋白表达下调；烧伤血清可以诱导单核细胞株Ｒａｗ２６４．７凋亡。结论：ＰＢＭＣ凋亡在严重烧伤的免疫功能紊乱方面起重要作用，而ｂｃｌ－２基因下调可能是重症烧伤早期ＰＢＭＣ细胞凋亡的分子机制之一；烧伤血清含有（或缺乏）某种物质，可以介导细胞凋亡。

Apoptosis of peripheral blood mononuclear cells in the early stage of severe burns in rats

AIM:To investigate the apoptosis and its alteration rule, molecular mechanism of peripheral blood mononuclear cells(PBMC) from severe burn rats. METHODS:The apoptosis of circulating PBMC ,monocytic cell Raw264.7 cultured in 10% burn serum and bcl -2 gene expression was detected by flow cytometric cell cycle analysis and DNA electrophoresis. RESULTS: Six hours after burn PBMC displayed characteristic apoptosis .Furthermore,the expression of bcl -2 gene was decreased effectively. Serum from burned rats can induce apoptosis in Raw264.7. CONCLUSIONS: Apoptosis may play a major role in thermal injury-related immunodeficiency.The decrease of bcl -2 protein might be one of the molecular mechanisms of apoptosis,and some humoral factor(s) in burn serum may play a role in the induction of apoptosis in Raw264.7 cell.