| 变形链球菌葡聚糖结合蛋白C基因特异片段的分子克隆 |
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| 引用本文： | 孙汉堂,吴补领,杨聚才,孙叶芳.变形链球菌葡聚糖结合蛋白C基因特异片段的分子克隆[J].牙体牙髓牙周病学杂志,2000,10(3):120-122. |
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| 作者姓名： | 孙汉堂 吴补领 杨聚才 孙叶芳 |
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| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
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| 摘 要： | 目的：克隆变形链球菌葡聚糖结合蛋白Ｃ基因（ｇｂｐＣ）编码序列的特异片段。方法：根据文献报道的ｇｂｐＣ序列设计并合成一对寡核苷酸引物，ＰＣＲ扩增位于ｇｂｐＣ编码序列１１０５～１５５４ｂｐ间的一段牧民片段，扩增产物经回收、酶切后，定向插入克隆载体ｐｕｃ１８中，连接产物转化感受态大肠杆菌ＤＨ５α，挑选阳性克隆，鉴定后进行序列测定。结果：测序结果与Ｓａｔｏ等报道的序列一致。结论：成功克隆了ｇｂｐＣ基因片段
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| 关 键 词： | 变形链球菌 葡聚糖结合蛋白 PCR 分子克隆 防龋 |
| 修稿时间： | 1999-10-13 |
The clonging and sequencing of a segment supposed to encode a glucan-binding domain of glucan-binding protein C of Streptococcus mutans |
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| SUN Han-tang,WU Bu-ling,YANG Ju-cai,et al School of Stomatology,The Fourth Military Medical University,Xi'an.The clonging and sequencing of a segment supposed to encode a glucan-binding domain of glucan-binding protein C of Streptococcus mutans[J].Chinese Journal of Conservative Dentistry,2000,10(3):120-122. |
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| Authors: | SUN Han-tang WU Bu-ling YANG Ju-cai School of Stomatology The Fourth Military Medical University Xi'an |
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| Institution: | SUN Han-tang,WU Bu-ling,YANG Ju-cai,et al School of Stomatology,The Fourth Military Medical University,Xi'an 710032 |
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| Abstract: | |
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| Keywords: | S mutans Glucan-binding protein PCR molecular cloning sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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