重组人血管生长素及其单克隆抗体的特性鉴定

目的 鉴定自制重组人血管生长素rhANG)及其单克隆抗体（mAb)。方法 采用凝胶扫描法分析rhANG的纯度；ELISA等方法检测抗rhANC mAb的特异性，鸡胚绒毛尿囊膜实验检测rhANG及其mAb的生物学活性，并与商品化标准品的免疫结合性进行对比研究。结果 rhANG的纯度达96％，具有促进血管生长的生物学活性；抗ANG mAb具有很好的特异性和抑制血管生长的活性；而且二者较标准品均具有良好的免疫结合活性。结论 该rhANG及其mAb可用于进一步的实验和临床应用研究。

The characterization of recombinant human an giogenin and its monoclonal antibody

Aim To detect the biological activity of the home made recombinant human angiogenin and its monoclonal antibody(mAb). Methods Gel scanning was used to analyze the purity of home made rhANG. ELISA and chicken CAM assay were used to detect the specificity and biological activity of ANG and anti ANG mAb, respectively. Immunobinding activities of home made rhANG and the standard rhANG were compared by routine ELISA. Results The purity of home made rhANG reached 96% and could induce the angiogenesis. The anti ANG mAb showed high specificity and inhibited effectively the proliferation of blood vessels induced by angiogenin. The home made and standard ANG, both products possessed good immunological binding activity. Conclusion The home made rhANG and its mAb can be used for further basic and clinical research.