| 接受嗅鞘细胞移植脊髓损伤大鼠电生理及后肢功能变化 |
| |
| 引用本文： | 王广志,刘明娜.接受嗅鞘细胞移植脊髓损伤大鼠电生理及后肢功能变化[J].中国组织工程研究与临床康复,2010,14(1). |
| |
| 作者姓名： | 王广志 刘明娜 |
| |
| 作者单位： | 1. 哈尔滨医科大学附属第二医院,医务部,黑龙江省哈尔滨市,150086
2. 哈尔滨医科大学附属第二医院,消化科,黑龙江省哈尔滨市,150086 |
| |
| 摘 要： | 背景:研究证实嗅鞘细胞有利于神经元存活,并可促进轴突再生.目的:探讨嗅鞘细胞移植治疗大鼠脊髓损伤的效果.方法:健康成年雌性SD大鼠40只,随机分为盐水对照组、细胞移植组,20只/组.另取10只SD大鼠用于嗅鞘细胞的分离培养.盐水对照组、细胞移植组大鼠均建立脊髓损伤模型,取双侧第8～10对肋间神经各2 cm,交叉植入脊髓缺损处(近端白质与远端灰质、远端白质与近端灰质),细胞移植组局部注射嗅鞘细胞2×10~6个,盐水对照组局部注射等量无菌生理盐水.通过体感诱发电位和运动诱发电位的检测,观察神经电生理恢复情况;BBB后肢运动功能评分结果;通过BDA顺行神经示踪,观察运动传导束恢复情况. 结果与结论:细胞移植组大鼠体感诱发电位及运动诱发电位的潜伏期、波幅明显优于盐水对照组(P < 0.01);细胞移植组大鼠BBB后肢运动功能评分较生理盐水组明显提高(P < 0.01);细胞移植组脊髓损伤区有较多BDA标记阳性神经纤维通过,其数量明显多于盐水对照组(P < 0.01).证实局部注射嗅鞘细胞可以较好地恢复大鼠脊髓损伤后的神经电生理及后肢运动功能.
|
| 关 键 词： | 脊髓损伤 嗅鞘细胞 移植 电生理 大鼠 运动功能 |
Electrophysiological changes and hindlimb motor function of a model rat with spinal cord injury following olfactory ensheathing cell transplantation |
| |
| Wang Guang-zhi,Liu Ming-na.Electrophysiological changes and hindlimb motor function of a model rat with spinal cord injury following olfactory ensheathing cell transplantation[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(1). |
| |
| Authors: | Wang Guang-zhi Liu Ming-na |
| |
| Institution: | Wang Guang-zhi1,Liu Ming-na21Department of Medical Branch,2Department of Digestion,Second Hospital Affiliated to Harbin Medical University,Harbin 150086,Heilongjiang Province,China |
| |
| Abstract: | BACKGROUND: Previous research has proved that olfactory ensheathing cells can promote neuronal survival and axonal regeneration. OBJECTIVE: To investigate the effect of olfactory ensheathing cell transplantation on the treatment of spinal cord injury of rats. METHODS: A total of 40 healthy adult female SD rats were randomly divided into control and cell transplantation groups, with 20 rats for each group. Ten additional SD rats were used for separation and culture of olfactory ensheathing cells. Spinal cord... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
