促红细胞生成素对慢性肾功能衰竭大鼠肾功能的影响

目的:研究促红细胞生成素(EPO)对慢性肾功能衰竭(CRF)大鼠肾功能的影响及其作用机制.方法:将5/6肾切除大鼠随机分为3组:Ⅰ组为假手术组;Ⅱ组为CRF组;Ⅲ组为给予促红细胞生成素的CRF组.第2次术后8周检测各组血压、尿蛋白、血清尿素氮、血肌酐、血红蛋白;观察肾组织病理改变,检测血清及肾组织中血红蛋白氧合酶 1(HO 1)活性;用免疫组化方法检测HO 1在肾脏中的表达.结果:Ⅲ组与Ⅱ组比较,血压、尿蛋白、血肌酐及尿素氮水平明显降低(P＜0.05),肾小球系膜增生及间质纤维化程度明显减轻(P＜0.05);HO 1活性检测显示,Ⅲ组大鼠血清中HO 1活性明显高于Ⅱ组(P＜0.05),免疫组化显示Ⅲ组大鼠肾组织中HO 1表达明显高于Ⅱ组(面密度、平均光度)(P＜0.05).结论:EPO使CRF大鼠肾功能得到改善,并使CRF大鼠血清及肾组织中HO 1表达及活性明显升高.

Studies of the Influence and Its Mechanism of Erythropoietin to the Renal Function in Chronic Renal Failure Rats

Objective: To investigate the influence and themechanism of erythropoietin to the renal function in chronic renal failure(CRF) rats. Methods:The 5/6 nephrectomized rats were randomly divided to three group. Group I : Shame operation group. Group II : CRT group. Group III: CKF group receiving erythropoietin. At the 8th week after second operation, blood pressure, urinary protein, serum creatinine, BUN and Hb were measured and renal pathological changes were observed. The activity of heme oxygenase-1(HO-1) in serum and renal tissue was also measured: the expression of HO-1 in kidney was examined by immunohistochemistry. Results : Compared with CRF group, the urinary protein, blood pressure, Scr and BUN of erythropoietin group were reduced significantly, and the glomerular mesangial proliferation and interstitial fibrosis were ameliorated significantly. The activity of HO-1 in the serum showed that group III was higher than group II ( P < 0.05). Immunohistochemistry showed that the expression of HO-1 in the kidney in group III was higher than group II ( P < 0.05) . Conclusion: EPO can ameliorate the renal function of CRF rats and can increase the expression and activity of HO-1 in serum and kidney of CRF rats.