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| 艾炷灸对D-半乳糖衰老小鼠大脑皮层细胞周期蛋白依赖性激酶抑制因子、视网膜母细胞瘤抑制蛋白及c-fos的影响 | |
| 作者姓名： | Du Y Zhao LH Wu HB Wang JS |
| 作者单位： | 1. 广西中医学院附属瑞康医院针灸科,南宁,530011 2. 广西中医学院第一附属医院针灸科,南宁,530023 3. 广西中医学院,南宁,530001 |
| 摘 要： | 目的:探讨艾炷灸治疗D-半乳糖衰老小鼠的可能机制。方法:雄性昆明小鼠60只随机分为生理盐水组、造模组、艾灸1组、艾灸2组、尼莫地平组,每组12只。皮下注射D-半乳糖造成小鼠衰老模型。艾灸1、2组分别选取小鼠"足三里""悬钟""关元""百会"行艾柱灸治疗,尼莫地平组给予尼莫地平灌胃。采用免疫组化法观察各组小鼠细胞周期蛋白依赖性激酶抑制因子(P16)、视网膜母细胞瘤抑制蛋白(pRb)及c-fos的表达。结果:造模组小鼠大脑皮质P16蛋白表达的神经元密度较生理盐水组增高(P0.01),艾灸1、2组及尼莫地平组较造模组降低(P0.01)。造模组小鼠pRb、c-fos蛋白表达的神经元密度较生理盐水组、艾炷灸、尼莫地平组降低(P0.01,P0.05)。其余组各指标比较差异无统计学意义(P0.05)。结论:艾炷灸"足三里""悬钟""关元""百会"可调控D-半乳糖衰老小鼠脑组织P16、pRb、c-fos蛋白的表达。 |
| 关 键 词： | 艾炷灸 衰老 大脑皮质 细胞周期蛋白P16 视网膜母细胞瘤 c-fos表达 |
Effects of moxibustion on the expression of cell cycle protein P 16 and retinoblastoma and c-fos in the cerebral cortex of senile mice |
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| Du Y,Zhao LH,Wu HB,Wang JS.Effects of moxibustion on the expression of cell cycle protein P 16 and retinoblastoma and c-fos in the cerebral cortex of senile mice[J].Acupuncture Research,2010,35(4):250-4, 276. | |
| Authors: | Du Yan Zhao Li-Hua Wu Hai-Biao Wang Jin-Sheng |
| Institution: | DU Yan,ZHAO Li-hua1,WU Hai-biao,WANG Jin-sheng2,YAO Ping2 (Department of Acu-moxibustion,the Affiliated Ruikang Hospital of Guangxi College of Chinese Medicine,Nanning 530011,China,1Department of Acu-moxibustion,the First Affiliated Hospital of Guangxi College of Chinese Medicine,Nanning 530023,2Guangxi College of Chinese Medicine,Nanning 530001) |
| Abstract: | Objective To observe the influence of moxibustion on the cyclin and cellular proliferin of the cerebral cortex in senile mice so as to explore its underlying mechanism in delaying aging. Methods Sixty male mice were randomly and equally divided into control,model,moxibustion of Zusanli (ST 36) and Xuanzhong (GB 39,M-ST 36-GB 39),moxibustion of Baihui (GV 20) and Guanyuan (CV 4,M-GV 20-CV 4),and medication groups. The aging model was established by subcutaneous injection of D-galactose for 42 days. Moxibusti... |
| Keywords: | Moxibustion Aging Cerebral cortex Cell cycle protein P 16 Retinoblastoma (Rb) c-fos expression |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |