依托咪酯对大鼠脑缺血再灌注损伤的保护作用及其机制

目的 探讨依托咪酯对大鼠脑缺血再灌注损伤的保护作用及其机制.方法 SD大鼠60只,随机分为对照组（S）、缺血再灌注组（IR）和依托咪酯组（Etom）,各20只.采用4-VO法建立大鼠全脑缺血再灌注模型.缺血再灌注后1d、3d、7d用免疫组化法测定海马CA1区神经元mPTP的表达.结果 与S组相比,IR组和Etomidate组海马CA1区神经元mPTP的表达明显上升（P〈0.01）,但Etom组海马CA1区神经元mPTP的表达低于IR组（P〈0.05）.组内各时间点表达水平差异无统计学意义（P〉0.05）.结论 依托咪酯对大鼠脑缺血再灌注损伤有一定的保护作用,可能是通过抑制mPTP的表达而发挥作用的.

Protective effects of etomidate against global brain ischemia-reperfusion injury in SD rats

Objective To investigate the protective effects and possible mechanisms of Et0midate against global brain is. chemia-reperfusion injury in SD rats. Methods Twenty-seven SD rats were randomly divided into three groups： sham operation group（S） ; IR group and Etomidate（Etom） group. Global brain ischemic model was established by 4-VO method. The expression of mPTP was determined by immunochemistry the 1 st day, 3th day, 7 th day after global brain ischemia-reperfusion injury. Results Compared with sham group, the expression of mPTP was significantly increased in IR group and Etom-group （P 〈 0. 01 ） ; but the expression of mPTP in Etom-group than IR group. Conclusion Peritonael injection of Etomidate before ischemia could protect the brain against IR injury in SD rats through an inhibition of mPTP expression.