| 野生型p53基因调控膀胱移行细胞癌中心体异常的实验研究 |
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| 引用本文： | 王明,王连渠,谭毅. 野生型p53基因调控膀胱移行细胞癌中心体异常的实验研究[J]. 中国医科大学学报, 2010, 39(2): 128-131 |
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| 作者姓名： | 王明 王连渠 谭毅 |
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| 作者单位： | 重庆医科大学,附属第一医院泌尿外科,重庆,400016 |
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| 摘 要： | 目的研究野生型p53基因抑制膀胱癌细胞中心体异常的作用。方法将野生型p53基因重组腺病毒载体转染人膀胱癌细胞T24,应用免疫荧光化学法检测膀胱癌细胞中心体异常变化情况。结果野生型p53基因可抑制膀胱移行细胞癌T24细胞中心体异常。结论p53基因可能是膀胱移行细胞癌中心体异常的一个重要调控因子,p53基因失活可能引起肿瘤细胞染色体不稳,从而促进肿瘤的发生和发展。
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| 关 键 词： | 野生型p53基因 膀胱癌细胞株 中心体异常 染色体不稳 |
Effect of Wild Type p53 Gene on Inhibiting the Centrosome Hyperamplification in Bladder Cancer Cell Line |
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| WANG Ming , WANG Lian-qu , TAN Yi. Effect of Wild Type p53 Gene on Inhibiting the Centrosome Hyperamplification in Bladder Cancer Cell Line[J]. Journal of China Medical University, 2010, 39(2): 128-131 |
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| Authors: | WANG Ming WANG Lian-qu TAN Yi |
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| Affiliation: | WANG Ming,WANG Lian-qu,TAN Yi(Department of Urology,The First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China) |
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| Abstract: | Objective To study the effect of wild type p53 gene on centrosome hyperamplification in bladder cancer cells.Methods A wild type p53 gene recombinant adenovirus vector AdCMVp53 was constructed,and then transfected into the human bladder cancer cell line T24.The cells were stained with the monoclonal antibody against pericentrin by indirect immunofluorescence method.The change of centrosome hyperamplification was observed under the fluorescence microspcope.Results Introduction of wild type p53 could suppress... |
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| Keywords: | wild type p53 gene bladder cancer cell line centrosome hyperamplification chromosome instability |
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