尼古丁对大鼠不同部位骨钙磷含量和碱性磷酸酶活性的差异影响

目的 分析尼古丁对大鼠牙槽骨和颌骨内钙磷含量以及碱性磷酸酶（ALP）活性的影响。方法 将20只5周龄健康雄性Wistar大鼠随机分为实验组和对照组，实验组每天腹腔注射尼古丁0.73 mg•kg-1，对照组腹腔注射等量生理盐水，3个月后处死取材。浓酸消化法检测牙槽骨和颌骨内钙磷含量，改良Reddi法检测牙槽骨和颌骨内ALP活性。采用SPSS 16.0统计软件分别行独立样本的t检验。结果 与对照组相比较，实验组牙槽骨内和颌骨内钙磷含量均明显减少，差异均有统计学意义（P<0.05）；ALP活性无明显变化（P>0.05）。实验组中牙槽骨与颌骨比较，骨钙磷含量和ALP活性差异无统计学意义（P>0.05）。结论 尼古丁下调大鼠牙槽骨和颌骨内钙磷含量，对ALP活性的影响不明显。

The effects of nicotine on bone calcium and phosphorus content and alkaline phosphatase activity in diffe-rent part of rat

Objective To analyze the effects of nicotine on the bone calcium and phosphorus content and alkaline phosphatase(ALP) activity in rat alveolar bone and mandible. Methods Twenty health male Wistar rats of five weeks of age were randomly assigned to two groups and received daily intraperitoneal injections for three months as follows: Saline solution for control group, nicotine 0.73 mg·kg-1·d-1 for experimental group. The bone calcium phosphorus content were detected by concentrated acid digestion method and the ALP activity was examined by improved Reddi method. Results Compared to the control group, bone calcium and phosphorus content was lower in the experimental group(P<0.05), ALP activity had no statistical significance(P>0.05). Bone calcium phosphorus and ALP activity in different parts had no statistical significance(P>0.05). Conclusion The nicotine reduces calcium phosphorus deposition of jaw bone, but has no obvious influence to ALP activity.