非小细胞肺癌患者外周血上皮细胞黏附分子和黏蛋白的诊断价值

目的: 研究非小细胞肺癌(non-small cell lung cancer, NSCLC)患者外周血中上皮细胞黏附分子(epithelial cell adhesion molecule, EpCAM) mRNA和黏蛋白1（MUC1） mRNA表达水平及临床意义。方法： 应用实时荧光定量PCR(qRT-PCR)技术检测NSCLC患者(n=45)与肺部良性疾病患者(n=21)外周血中EpCAM mRNA和MUC1 mRNA的表达情况；分析NSCLC患者EpCAM mRNA和MUC1 mRNA阳性表达率与临床病理特征的相关性。结果： NSCLC患者外周血中EpCAM mRNA和MUC1 mRNA的表达水平分别为4.42±0.56,2.30±0.32;肺部良性疾病患者外周血中EpCAM mRNA和MUC1 mRNA的表达水平分别为2.48±0.26，1.61±0.06；两者相比，差异均有统计学意义（EpCAM mRNA， P=0.025;MUC1 mRNA， P=0.018）。NSCLC患者外周血中EpCAM mRNA和MUC1 mRNA阳性表达率分别与肺部良性疾病患者比较，差异均有统计学意义(P均=0.001)。NSCLC患者外周血中这两种基因表达的阳性率与其年龄、性别及病理类型无明显相关性；而与TNM分期显著相关，Ⅲ+Ⅳ期NSCLC患者外周血中EpCAM mRNA和MUC1 mRNA阳性表达率明显高于Ⅰ+Ⅱ期患者(EpCAM mRNA,P=0.021;MUC1 mRNA,P=0.005)。结论： 检测外周血中EpCAM mRNA和MUC1 mRNA的表达有助于NSCLC的诊断，尤其对血液微转移的检出具有重要的临床参考价值。

Diagnostic value of epithelial cell adhesion molecule and mucin1 in peripheral blood from patients with non-small cell lung cancer

Objective: To study the clinical significance of mRNA expressions of epithelial cell adhesion molecule(EpCAM) and mucin1(MUC1) in peripheral blood from patients with non-small cell lung cancer(NSCLC). Methods: The mRNA expressions of EpCAM and MUC1 in peripheral blood samples from 45 patients with NSCLC and 21 patients with benign pulmonary disease were detected by quantitive real-time polymerase chain reaction (qRT-PCR). Correlations between the two mRNA expressions and clinicopathologic characteristics of NSCLC patients were analysed. Results: The levels of mRNA expressions of EpCAM and MUC1 in patients with NSCLC were significantly higher than those in patients with benign pulmonary disease(4.42±0.56 vs 2.48±0.26, P=0.025 for EpCAM; 2.30±0.32 vs 1.61±0.06, P=0.018 for MUC1). There were significant differences between NSCLC patients and patients with benign pulmonary disease with regard to the positive rates of mRNA expressions of EpCAM and MUC1(both P＜0.01). The two mRNA expressions were not significantly correlated with age, gender and histopathologic type, but correlated with TNM staging of tumor; and the positive rates of mRNA expressions of EpCAM and MUC1 in peripheral blood from patients with stage Ⅰ+Ⅱ NSCLC were higher than those of patients with stage Ⅲ+Ⅳ NSCLC(P=0.021 and P=0.005, respectively). Conclusion: Detection of EpCAM mRNA and MUC1 mRNA in peripheral blood could be favourable for the clinical diagnosis of NSCLC, especially for detection of blood micrometastasis.