| 穿山龙总皂苷的HPLC指纹图谱研究 |
| |
| 引用本文： | 张园园,李静,潘激扬,陈晓辉,毕开顺.穿山龙总皂苷的HPLC指纹图谱研究[J].中国药房,2012(27):2555-2557. |
| |
| 作者姓名： | 张园园 李静 潘激扬 陈晓辉 毕开顺 |
| |
| 作者单位： | [1]北京中医药大学中药学院,北京100102 [2]第三军医大学药学院,重庆400038 [3]沈阳药科大学药学院,沈阳110016 |
| |
| 摘 要： | 目的:建立穿山龙总皂苷的高效液相色谱(HPLC)指纹图谱,为其质量评价提供依据。方法:测定9批不同来源的穿山龙样品。色谱柱为Kromasil C8(250mm×4.6mm,5μm),流动相为水-乙腈(梯度洗脱),流速为1.0mL·min-1,检测波长为203nm,柱温为30℃。结果:共标示出穿山龙皂苷类化合物17个特征指纹峰;不同来源的穿山龙总皂苷色谱峰峰面积比值有一定的差异。结论:HPLC指纹图谱分析法能简便、快速地区分不同来源的穿山龙,为全面控制穿山龙药材的质量提供依据。
|
| 关 键 词： | 穿山龙 皂苷 反相高效液相色谱法 指纹图谱 |
HPLC Fingerprint of Total Saponins from Dioscorea nipponica |
| |
| ZHANG Yuan-yuan,PAN Ji-yangSchool of TCM,Beijing University of TCM,Beijing,China LI JingCollege of Pharmacy,Third Military Medical University,Chongqing,China CHEN Xiao-hui,BI Kai-shun.HPLC Fingerprint of Total Saponins from Dioscorea nipponica[J].China Pharmacy,2012(27):2555-2557. |
| |
| Authors: | ZHANG Yuan-yuan PAN Ji-yangSchool of TCM Beijing University of TCM Beijing China LI JingCollege of Pharmacy Third Military Medical University Chongqing China CHEN Xiao-hui BI Kai-shun |
| |
| Institution: | (School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China) |
| |
| Abstract: | OBJECTIVE： To establish the HPLC fingerprints for total saponins from Dioscorea nipponica, and to provide refer- ence for quality evaluation of it. METHODS： 9 batches of D. nipponica collected from different regions were determined. The sepa- ration was performed on Kromasil C8（250mm×4.6mm,5μm） column using water-acetonitrile （gradient elution） as mobile phase at flow rate of 1.0 mL·min^-1. The UV detection wavelength was set at 203 mn and the column temperature was 30 ℃. RESULTS： 17 peaks were identified as the characteristic fingerprints of saponins from D. nipponica and the ratio of peak area of different sam- pies were different. CONCLUSION： The method is suitable to differentiate D. nipponica from different regions conveniently, and can provide reference for quality control of D. nipponica completely. |
| |
| Keywords: | Dioscorea nipponica Saponins RP-HPLC Fingerprint |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
