HPLC法同时测定傣药灯台叶中鸭脚树叶碱、齐墩果酸和熊果酸的含量

目的:建立同时测定傣族传统药物灯台叶中鸭脚树叶碱、齐墩果酸和熊果酸含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent ZORBAX Eclipse SB-C18(250mm×4.6mm,5μm),流动相为乙腈-pH7.6的磷酸盐缓冲液(梯度洗脱),检测波长为220nm。结果:鸭脚树叶碱、齐墩果酸和熊果酸3种成分的检测浓度分别在0.01～0.20mg·mL-1(r=0.9997)、0.06～1.20mg·mL-1(r=0.9996)、0.10～2.00mg·mL-1(r=0.9998)范围内与各自峰面积积分值呈良好线性关系;平均加样回收率依次为102.0%、101.1%、99.3%,RSD分别为1.84%、2.00%、0.91%(n均为6)。5批灯台叶中鸭脚树叶碱的含量在0.085%～0.122%之间,齐墩果酸含量在0.517%～0.582%之间,熊果酸含量在1.71%～1.85%之间。结论:本方法简便、准确、精密度高、重复性好,可同时测定灯台叶中鸭脚树叶碱、齐墩果酸和熊果酸的含量,可为灯台叶的质量控制提供科学依据。

Simultaneous Determination of Picrinine, Oleanolic Acid and Ursolic Acid in Leaves of Dai Medicine Alstonia scholaris

OBJECTIVE： To establish a method for the simultaneous determination of picrinine, oleanolic acid and ursolic acid in leaves of Dai medicine Alstonia scholaris. METHODS： HPLC method was adopted. The samples were separated on Agilent ZORBAX Eclipse SB-C18（250 mm×4.6 mm, 5 μm）colunm with the mobile phase consisted of acetonitrile-pH 7.6 phosphate buffer （gradient elution）. The detection wavelength was set at 220 nm. RESULTS： The linear ranges were 0.01-0.20 mg· mL^-1 for picrinine（r=0.999 7）,0.06-1.20 mg·mL^-1 for oleanolic acid （r=0.999 6） and 0.10-2.00 mg.mL-1 for ursolic acid （r=0.999 8）, respectively. The average recovery was 102.0% （RSD=l.84%）, 101.1% （RSD=2.00%）, 99.3% （RSD=0.91%）, respectively （n=6）. The contents of picrinine, oleanolic acid and ursolic acid in five batches ranged 0.085%-0.122%, 0.517% -0.582%, 1.71% -1.85%, respectively. CONCLUSION： The method is simple, accurate, precise and reproducible, which can be used for the simultaneous determination of picrinine, oleanolic acid and ursolic acid in leaves of A. scholaris. It provides scientific basis for quality control of the leaves ofA. scholaris.