毛细管GC法测定南五味子多糖的单糖组成

目的:研究南五味子多糖中单糖的种类和组成比例。方法:以2moL.L-1硫酸溶液水解南五味子多糖,用吡啶溶解干燥的水解产物,硅烷化衍生;采用毛细管气相色谱法测定南五味子多糖的单糖组成:色谱柱为SE-30石英毛细管(30m×0.25mm×0.25μm),进样口温度为220℃,检测器温度为230℃,柱温为195℃。结果:南五味子多糖中含有甘露糖、木糖、半乳糖、果糖、葡萄糖和半乳糖醛酸6种单糖成分,已确定的6种单糖的摩尔比为19.78:2.75:0.149:1:0.381:0.127。结论:该方法简便、灵敏,可以准确测定出南五味子多糖中含有的各种单糖。

Analysis of Monosaccharides Composition in Polysaccharide from Kasura marmorata by Capillary Gas Chromatography

OBJECTIVE： To study the types and proportion of monosaccharides in Kasura marmorata polysaccharide. METHODS： K. marmorata polysaccharide was hydrolyzed with 2 moL-L-1 sulfuric acid. The dried hydroxlyslate was dissolved in pyridine, and derived by silane. The monosaccharides in K. marmorata polysaccharide were determined by capillary GC. The determination was performed on SE-30 （30 m × 0.25 mm× 0.25 μm） capillary column. Injection and detector temperature was 220 ℃ and 230 ℃ respectively. The column temperature was 195 ℃. RESULTS： K. marmorata polysaccharide made up of six monosaccha- rides, such as mannos, exylose, galactose, fructose, glucose, galacturonic acid. The mole proportion of mannose-xylose-galactose-fructose-glucose-galacturonic acid was 19.78 ： 2.75 ： 0.149 ： 1 ： 0.381 ： 0.127. CONCLUSION： The method reported in this paper is simple and sensitive, which can be used for determination of monosaccharides composition in K. marmorata polysaccharide.