基于共有峰率和变异峰率双指标序列分析法的芡实红外指纹图谱研究

目的:建立不同产地芡实的红外指纹图谱。方法:利用共有峰率和变异峰率2个指标,以不同产地芡实的红外指纹图谱为标准,计算出17个样品间的共有峰率和变异峰率,按照其大小建立了不同的共有峰率和变异峰率双指标序列分析法,研究各产地芡实的异同。结果:产地相近的S13与S12、S14之间,S2与S3之间有很高的共有峰率(均不低于82.4%)和很低的变异峰率(均不超过21.4%);产地不同但品种一致的S9与S1、S15之间,S4与S6之间有不高的共有峰率(均不超过52.4%)和不低的变异峰率(均不低于33.3%);地理位置相距较远的S16与S8、S6之间,S17与S4、S8之间有较低的共有峰率(均不超过47.6%)和较高的变异峰率(均不低于50.0%);产地不同且采收期较近,造成了S10与其他产地样品相比有着极低的共有峰率(均不超过35.7%)和极高的变异峰率(几乎均高于100%)。结论:双指标序列分析法结合红外指纹图谱能够指导判别不同产地的芡实,简便、快捷,可为芡实等水生植物药材的质量评价提供一种新方法。

Study on IR Fingerprint of Euryale ferox Based on Dual-index Sequence Analysis of Common and Variant Peak Ratios

OBJECTIVE： To establish IR fingerprint of Euryale ferox collected from different regions. METHODS： Using common peak ratio index and variant peak ratio index, based on IR fingerprint of Euryale ferox from different regions, common peak ratio and variant peak ratio of 17 samples were calculated respectively to set up a new method called dual-index sequence analysis for distinguishing samples from different regions. RESULTS： It showed sample S13 and S12, S14, sample S2 and S3 were the most similar samples with higher common peak ratio（≥82.4% ） and lower variant peak ratio （ ≤21.4% ）. Sample S9 and S1, S15, sample S4 and S6 from different regions but of same specie, were average with similar common peak ratio （≤52.4%） and variant peak ratio （/〉33.3%）. However, sample S16 and S8, S6, sample S17 and S4, S8 from the farther regions, were of significant disparity with lower common peak ratio（ ≤47.6% ） and higher variant peak ratio （ ≥50.0% ）. In addition, sample S10 and other samples from different region but at closer collecting time, which resulted in much lower common peak ratio （ ≤35.7% ） and much higher variant peak ratio（ ≥ 100.0% ）. CONCLUSION： The combination of dual-index sequence analysis and IR fingerprint gave a simple and efficient guidance in distinguishing Euryale ferox collected from different regions, which offer a new approach to quality evaluation of Euryale ferox and other aquatic plants