注射用羟基红花黄色素A对大鼠脑神经元线粒体的保护作用研究

目的:研究注射用羟基红花黄色素A对大鼠脑神经元线粒体的保护作用。方法:采用线栓法复制大鼠脑缺血再灌注模型,实验分为假手术(等容生理盐水)、模型(等容生理盐水)、尼莫地平(1.0mg.kg-1)和羟基红花黄色素A高、中、低剂量(10.24、5.12、2.56mg.kg-1)组。尾iv给药,每天1次,连续3d。测定大鼠血清中血小板活化因子(PAF)含量、脑海马组织Na+-K+-ATP酶、Ca2+-ATP酶和Mg2+-ATP酶活性;观察电镜下灰质部位神经元线粒体超微结构。结果:与模型组比较,羟基红花黄色素A高、中、低剂量组大鼠血清中PAF的含量显著降低,海马组织Na+-K+-ATP酶、Ca2+-ATP酶和Mg2+-ATP酶含量显著提高(P<0.01);神经元线粒体结构较完整,线粒体的破坏明显比模型组轻。结论:注射用羟基红花黄色素A具有明显的脑线粒体保护作用。

Protective Effect of HSYA Freeze-dried Powder on Brain Neuronal Mitochondria of Rats

OBJECTIVE： To study the protective effect of HSYA freeze-dried powder on brain neuronal mitochondria of rats. METHODS： Cerebral ischemia-reperfusion model was induced by suture method. Model rats were divided into 5 groups, i.e. sham operation group （constant volume normal saline）, model group （constant volume normal saline）, nimodipine group（1.0 mg.kg-1）, HSYA high-dose, medium-dose and low-dose groups （10.24 mg-kg-1, 5.12 mg. kg-1, 2.56 mg. kg-1）. They were given relevant drugs intravenously via tail once a day for consecutive 3 days. The content of PAF in serum and the activity of Na^＋-K＋-ATP enzyme, Ca2＋-ATP enzyme and Mg^2＋-ATP enzyeme in hippocampus were all determined. We observed ultrastructure of mitochondrial in gray area with electron microscope. RESULTS： Compared with model group, the content of PAF in serum of HSYA high-dose, medium-dose and low-dose groups could decrease significantly, and the activity of Na^＋-K＋-ATP enzyme, Ca^2＋-ATP enzyme and Mg^2＋-ATP enzyme in hippocampus could increase significantly（P〈0.01）. Mitochondrial structure was more complete and mitochondrial damaging in HSYA groups was slighter than that in model group. CONCLUSION： HSYA freeze-dried powder has a significant protective effect on brain mitochondria.