褐藻多糖硫酸酯精提物对慢性肾衰模型大鼠的保护作用研究

目的:研究褐藻多糖硫酸酯精提物(RFSE)对慢性肾衰模型大鼠的保护作用。方法:70只大鼠随机分为正常对照(1%羧甲基纤维素钠)、模型(1%羧甲基纤维素钠)、地塞米松(0.1mg.kg-1)、褐藻多糖硫酸酯(FS,100mg.kg-1)和RFSE高、中、低剂量(100、50、25mg.kg-1)组。灌胃365mg.kg-1腺嘌呤(每天1次,连续20d)以复制慢性肾衰模型。模型复制成功后,除正常对照组外,各组均灌胃给药,每天2次,连续25d,末次给药后测定24h尿量、尿蛋白、红细胞计数(RBC)、血红蛋白(Hb)、肌酐(Scr)、尿素氮(BUN)、Ca2+和P3+,并行肾脏组织形态学观察。结果:高、中、低剂量RFSE均能减少模型大鼠24h尿量、尿蛋白的排泄,降低血清Scr、BUN和P3+的水平(P<0.01或P<0.05);高、中剂量RFSE还可减轻肾衰模型大鼠的肾脏病理改变(P<0.01)。结论:RFSE对腺嘌呤所致的大鼠慢性肾衰有明显的保护作用。

Protective Effect of Refined Fucoidan Sulfate Extraction on the Chronic Renal Failure in Rats

OBJECTIVE： To investigate the protective effect of refined fucoidan sulfate extraction （RFSE） on chronic renal failure （CRF） in rats. METHODS： 70 SD rats were randomly divided into 7 groups, i.e. normal control group （1% sodium carboxymethylceUulose）, model group （1% sodium carboxymethylcellulose）, dexamethasone group （0.1 mg.kg^-1）, fucoidan sulfate （FS, 100 mg.kg^-1） group and RFSE high, middle and low-dose groups （100, 50, 25 mg.kg^-1）.CRF model was built successfully by adenine 365 mg.kg^-1 intragastrically once a day for consecutive 20 day. Those groups were feed drugs for successive 25 days twice a day intragastrically. The 24 h urine volume, urine protein, RBC, Hb, Set, BUN, Ca2＋ and p3＋ were determined after the last administration, Histomorphology of kidney was observed. RESULTS： It was observed that urine volume and protein in 24 hours, and the levels of Scr, BUN, and p3＋ in high, middle, and low-dose of RFSE groups were significantly lowered （P〈0.01 or P〈0.05）. Moreover, the pathological changes were improved in the high and medium doses of RESE groups（P〈0.01）. CONCLUSION： RFSE has an obviously protective effect on the adenine induced CRF in rats. KEY WORDS Refined fucoidan extraction; Adenine; Chronic renal failure