胶原蛋白在咬肌附着及再附着界面的表达

目的　探讨山羊咬肌与下颌骨皮质骨面和松质骨面再附着中胶原在肌-骨界面的表达及意义。方法　以9只成年山羊为研究对象,其中1只为正常对照。将实验组山羊双侧下颌骨咬肌附着从骨面剥离,附着区骨面平均分为二部分,分别保留原骨面或去除皮质骨,暴露松质骨,咬肌原位缝合。术后2、4、8、12周采集标本,采用免疫组织化学ABC法检测两种肌-骨界面Ⅰ、Ⅱ、Ⅲ型胶原的表达情况。结果　免疫组化观察表明,Ⅰ、Ⅱ、Ⅲ型胶原在愈合过程中的表达不同,但两种骨面的肌-骨界面胶原表达无明显差别。结论　再附着界面胶原表达随时间变化而与附着骨面结构无关。

Expression of Collagens in Reattached Masseter Muscles to Mandibles Following a Surgical Detachment

OBJECTIVE: The aim of this study was to investigate the expression of collagen in the process of masseter muscle reattachment to the cortical and cancellous bones of mandible. METHODS: A total of nine adult goats were used in the study. One was the control. The other eight were treated with bilateral detachment of the masseter muscles. The biopsies of bone and muscle were taken at 2, 4, 8 and 12 weeks after the operation. The characteristics of the healing muscle-bone interfaces were examined using immunohistochemical techniques. RESULTS: Immunohistochemical analysis illustrated that the locations of collagen type I, II and III were different during the healing process, but similar in the cortical and cancellous bones. CONCLUSION: This study demonstrates that the distribution of the three types of collagens at the muscle-bone interfaces is associated with time, but not related with their locations.