脑缺血再灌注损伤后神经细胞增殖相关因子基因的表达

目的：观察大鼠脑缺血再灌注后神经细胞巢蛋白、干细胞因子（SCF）和神经细胞粘附分子（NCAM）基因的表达。方法：成年SD大鼠，以线栓法建立大脑中动脉缺血再灌注模型，随机分为缺血1．5h再灌注2h～14d组和假手术组。原位杂交技术检测脑缺血再灌注后脑组织巢蛋白SCF和NCAM mRNA的表达。结果：脑缺血再灌注后，巢蛋白mRNA表达在大部分时相点，均明显高于假手术组。SCF mRNA的表达除早期时相点外，均明显高于假手术组。NCAM mRNA于再灌注2h后开始表达，12h～1d达高峰，7d后逐渐减少，14d降至假手术组水平。结论：脑缺血再灌注后SCF mRNA表达可能具有促进神经干细胞增殖作用，NCAM表达可能参与了损伤后脑组织的修复过程。

Gene expression of nerve cell proliferation associated factors following cerebral ischemia reperfusion in rats

Objective: To study the expressions of Nestin,stem cell factor(SCF) and neural cell adhesion molecule(NCAM) in neurons after ischemia reperfusion injury in rat brain.Methods: Thirty-six adult female SD rats were induced by intraluminal middle cerebral artery(MCA) occlusion for 1.5()h and reperfusion for 2()h,6()h,12()h,1()d,2()d,3()d,7()d and 14()d treated groups and sham-operated group(n=4)] with a nylon monofilament suture.In situ hybridization was used to examine the expression of Nestin,SCF and NCAM mRNA.Results: Nestin mRNA expression in cortex and striatum increased markedly in ischemic hemisphere at most times compared to sham-operated group.SCF mRNA expression in cortex and striatum increased significantly in ischemic hemisphere except for early times compared to sham-operated group.NCAM mRNA expression was observed after reperfusion(2()h),peaked at reperfusion 12()h in cortex and 1()d in striaum,and decreased to the basic level of sham-operated group at reperfusion 14()d.Conclusion: SCF expression may enhance neural stem cells proliferation following ischemia reperfusion.The increasing NCAM expression may play an important role in the repair of injured brain tissue.